Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Utilizing Resazurin Fluorescence to Monitor Kidney Function and Organic Anion Transporter Activity

Award Number: R01DK146470
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/01/2026
PERIOD OF PERFORMANCE END DATE: 01/31/2031

Group Awards By:

View Award Description

Utilizing Resazurin Fluorescence to Monitor Kidney Function and Organic Anion Transporter Activity - ABSTRACT A comprehensive and accurate assessment of kidney function is essential for managing renal diseases, but current diagnostic methods often provide an incomplete evaluation. Standard tests, such as glomerular filtration rate (GFR) measurements and proteinuria, primarily assess glomerular function and renal perfusion, offering limited insight into tubular health. Furthermore, compensatory increases in single-nephron filtration can sustain GFR even in the presence of nephron loss, often masking disease progression. These limitations are especially apparent in acute kidney injury (AKI), where GFR may return to normal even as tubular dysfunction persists, obscuring the transition to chronic kidney disease (CKD). Direct assessment of tubular function can provide valuable prognostic information by complementing existing diagnostic methods. To this end, we recently identified resazurin dye as a novel, non-invasive sensor for evaluating tubular function and nephron mass. Following intravenous injection, resazurin is selectively taken up by tubular cells, reduced to fluorescent metabolites, and excreted in urine, offering a GFR-independent measure of tubular secretion. In murine models of severe AKI and subclinical nephron loss (unilateral nephrectomy and genetic renal hypoplasia), urinary excretion of resazurin metabolites sensitively reflected both tubular dysfunction and nephron mass loss. Based on these findings, we propose that resazurin provides a rapid, sensitive, and GFR-independent method for quantifying tubular function, nephron mass, and renal reserve following kidney injury. This hypothesis is based on the premise that while nephron loss can lead to compensatory increases in single-nephron filtration to maintain GFR, tubular secretion is less adaptable, making it a more reliable indicator of tubular function and nephron mass. This proposal aims to investigate the tubular transport and metabolism of resazurin (Aim 1) as well as its effectiveness and sensitivity (Aim 2) in quantifying nephron mass loss in murine and porcine models of AKI. The results will provide proof-of-principle data to support clinical translation, facilitating earlier detection of nephron loss, enabling the identification of high-risk AKI patients, and improving monitoring of recovery from AKI. Ultimately, this work has the potential to transform the way we assess kidney function in both preclinical and clinical settings by providing a non-invasive and precise tool for detecting early tubular dysfunction and nephron loss.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2027 ( Subtotal = $0 )
2027	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	10/20/2026	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $0

Issue Date FY: 2026 ( Subtotal = $780,185 )
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	006	2	9/15/2026	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$99
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	003	2	8/24/2026	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$760,644
2026	2026	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	1	8/13/2026	NEW	-$769,736
2026	2026	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	2/1/2026	NEW	$789,350
2026	2026	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	005	1	9/15/2026	NEW	$99
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	W5510 FRANKS MELVILLE MEMORIAL LIBRARY	STONY BROOK	NY	11794	SUFFOLK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	004	2	8/25/2026	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$11,946
2026	2026	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	002	1	8/23/2026	NEW	$11,873
														Subtotal = $780,185

Grand Total All Awards = $780,185

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Utilizing Resazurin Fluorescence to Monitor Kidney Function and Organic Anion Transporter Activity

Award Number: R01DK146470

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/01/2026

PERIOD OF PERFORMANCE END DATE: 01/31/2031

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer