Monday, September 15, 2025 9/15/2025

A spatial view of hematopoietic regeneration dynamics in the bone marrow

Award Number: R01DK143671
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2024
PERIOD OF PERFORMANCE END DATE: 07/31/2029

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A spatial view of hematopoietic regeneration dynamics in the bone marrow - Project Summary: The regeneration of blood and immune cells relies on hematopoietic stem and progenitor cells (HSPCs) that reside in the bone marrow. HSPCs not only sustain homeostasis of the blood pool by constantly producing the precise amounts and types of blood cells as needed, but they also respond rapidly to injuries such as bleeding or infection. During these processes, HSPCs constantly sense and react to signals from various bone marrow cell types. Their spatial proximity to the sources of these signals change as they migrate during differentiation and proliferation within the tightly packed bone marrow. The spatial organization of individual HSPCs forms a dynamic landscape that modulates their intercellular communication. In the proposed project, we will study the dynamic spatial configuration of HSPCs during hematopoiesis and its impact on intercellular signaling, proliferation, differentiation, and injury response. The central hypothesis of this proposal is that cell fate transitions during hematopoiesis are associated with the migration of HSPCs through distinct bone marrow micro-environments where they are exposed to signals that promote their expansion and differentiation. We propose to use MEMOIR and seqFISH technologies to (1) map the spatial organization of HSPCs in the bone marrow, (2) investigate the dynamic changes of HSPCs’ spatial positioning during hematopoiesis and upon the demand of a specific blood cell type, and (3) determine the impact of HSPCs’ spatial context on their intercellular signaling and fate choices. Our findings will introduce a novel, spatial perspective of blood and immune cell regeneration and significantly impact many biomedical fields including immunology, hematology, tissue engineering, aging, and cancer. Moreover, this work will provide an experimental and conceptual framework for analyzing spatially defined intercellular communication in other tissue contexts.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $1,614,464 )
2025	2025	UNIVERSITY OF SOUTHERN CALIFORNIA	3720 S FLOWER ST FL 3	LOS ANGELES	CA	90033	LOS ANGELES	USA	Trans-NIH Research Support	000	2	7/30/2025	NON-COMPETING CONTINUATION	$1,614,464
														Subtotal = $1,614,464

Issue Date FY: 2024 ( Subtotal = $1,630,964 )
2024	2024	UNIVERSITY OF SOUTHERN CALIFORNIA	3720 S FLOWER ST FL 3	LOS ANGELES	CA	90033	LOS ANGELES	USA	Trans-NIH Research Support	000	1	9/6/2024	NEW	$1,630,964
														Subtotal = $1,630,964

Grand Total All Awards = $3,245,428

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A spatial view of hematopoietic regeneration dynamics in the bone marrow

Award Number: R01DK143671

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2024

PERIOD OF PERFORMANCE END DATE: 07/31/2029

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