Tuesday, August 19, 2025 8/19/2025

Cell type-specific complement activation and glial reactivity in male and female mice with dietary obesity

Award Number: R01DK134202
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/24/2023
PERIOD OF PERFORMANCE END DATE: 04/30/2028

Group Awards By:

View Award Description

Cell type-specific complement activation and glial reactivity in male and female mice with dietary obesity - PROJECT SUMMARY Neuroinflammation is a shared feature of obesity and dementia, but the processes that initiate and perpetuate glial reactivity are not well understood,. Several recent studies, including from our lab, have outlined signaling cascades linking peripheral and central inflammation in obesity in males, but vulnerability to obesity-induced metabolic pathology is sexually dimorphic, and susceptibility to neuroinflammation follows similar patterns. Adult females exhibit stronger innate immunity, and evidence from clinical and experimental studies suggests that this dimorphism involves sex differences in activation of complement. However, current understanding of tissue- specific complement activation remains rudimentary due to the scarcity of approaches for cell type-specific manipulation. To this end, we generated transgenic mice with inducible, cell type-specific deletion of the endogenous complement inhibitor Crry, and will use this model to distinguish between target cells and 'bystander' populations in the adult brain. After determining whether selective activation of complement is sufficient for neuroinflammation, we will examine the role of sexually dimorphic complement activation as a mechanism for differential vulnerability to synaptic and behavioral dysfunction on obesogenic diets. These themes will be addressed using state-of-the-art methodology, including newly developed strategies for high-throughput morphometric analysis of glial cells in cleared brains and region-specific manipulation of glial gene expression.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $364,658 )
2025	2025	AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC	AUGUSTA UNIVERSITY 1120 15TH STREET	AUGUSTA	GA	30912	RICHMOND	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	5/7/2025	NON-COMPETING CONTINUATION	$364,658
														Subtotal = $364,658

Issue Date FY: 2024 ( Subtotal = $365,384 )
2024	2024	AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC	AUGUSTA UNIVERSITY 1120 15TH STREET	AUGUSTA	GA	30912	RICHMOND	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	5/7/2024	NON-COMPETING CONTINUATION	$365,384
														Subtotal = $365,384

Issue Date FY: 2023 ( Subtotal = $394,157 )
2023	2023	AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC.	1120 15TH ST STE CJ3301	AUGUSTA	GA	30912	RICHMOND	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	7/21/2023	NEW	$394,157
														Subtotal = $394,157

Grand Total All Awards = $1,124,199

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Cell type-specific complement activation and glial reactivity in male and female mice with dietary obesity

Award Number: R01DK134202

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/24/2023

PERIOD OF PERFORMANCE END DATE: 04/30/2028

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer