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The gut endoderm: origin, formation and fate

Award Number: R01DK127821
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/01/2021
PERIOD OF PERFORMANCE END DATE: 01/31/2025

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The gut endoderm: origin, formation and fate - PROJECT SUMMARY / ABSTRACT The gut endoderm is the precursor tissue of the respiratory and digestive tracts, and their associated visceral organs. In this project we will use a suite of state-of-the-art technologies to address fundamental questions focused on mechanisms of mammalian gut endoderm cell lineage commitment and tissue morphogenesis, as well as probing the developmental origin and fate of cells that comprise the gut endoderm, using the mouse model. Our previous studies have provided a paradigm-shift in our understanding of the origin and mechanism of formation of the gut endoderm. Using fate mapping, live imaging and more recently single-cell transcriptomic methods, we demonstrated that the gut endoderm of mice comprises cells of two distinct developmental origins; embryonic definitive endoderm, and extra-embryonic visceral endoderm. The dual origin of the gut endoderm challenges the prevailing view of germ layer formation in mammals which posits that endoderm, along with mesoderm and ectoderm, derives solely from pluripotent epiblast. We have also shown that the gut endoderm forms through a novel intercalation mechanism and identified SOX17 as a critical regulator of this process. The broad aim of this project is to use molecular, genomic, embryological and imaging techniques to investigate fundamental open questions pertaining the origin, formation and fate of the gut endoderm in mammals. Specific Aim 1 will investigate the dynamic cellular behaviors driving gut endoderm morphogenesis. Specific Aim 2 will probe the mechanism(s) by which the SOX17 transcription factor drives gut endoderm cell fate specification, tissue morphogenesis and communication between embryonic definitive endoderm and extra-embryonic visceral endoderm cells as they form a congruent epithelium. Specific Aim 3 will determine whether descendants of extra-embryonic visceral endoderm cells persist throughout embryonic development and whether they will ultimately contribute to the endodermal organs of the adult. A rigorous understanding of the normal gut endoderm, encompassing its origin, formation and fate, will underpin logical efforts to direct the differentiation of cells into endoderm identities, generate bona fide endodermal organoids for development and disease modeling and screening, understand disease progression and design new therapeutic strategies for these vital organ systems when they fail.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	4/24/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $626,223 )
2024	2024	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVENUE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	4	5/10/2024	NON-COMPETING CONTINUATION	$39,137
2024	2024	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVENUE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	1/5/2024	NON-COMPETING CONTINUATION	$587,086
														Subtotal = $626,223

Issue Date FY: 2023 ( Subtotal = $652,318 )
2023	2023	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	1/20/2023	NON-COMPETING CONTINUATION	$652,318
														Subtotal = $652,318

Issue Date FY: 2022 ( Subtotal = $652,318 )
2022	2022	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	2	3/29/2022	NON-COMPETING CONTINUATION	$65,233
2022	2022	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	1/19/2022	NON-COMPETING CONTINUATION	$587,085
														Subtotal = $652,318

Issue Date FY: 2021 ( Subtotal = $648,756 )
2021	2021	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	1/29/2021	NEW	$648,756
														Subtotal = $648,756

Grand Total All Awards = $2,579,615

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The gut endoderm: origin, formation and fate

Award Number: R01DK127821

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/01/2021

PERIOD OF PERFORMANCE END DATE: 01/31/2025

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