Tuesday, November 4, 2025 11/4/2025

Mass Spectrometric Studies of Neuropeptides in Feeding

Award Number: R01DK071801
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2006
PERIOD OF PERFORMANCE END DATE: 02/28/2027

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Mass Spectrometric Studies of Neuropeptides in Feeding - PROJECT SUMMARY/ABSTRACT Feeding behavior is critical for animal survival, and is also a fundamental aspect of energy homeostasis. This process is regulated by highly complex neurochemical pathways involving a multitude of neuropeptides and biogenic amines. Despite decades of work on individual neurochemical systems, the general organizational principles underlying neuromodulation are still poorly understood. This is mainly due to the fact that modulation of neural circuit has so far been studied primarily one modulator at a time without the knowledge about co-modulation of networks. The latter information would require the development of sensitive and selective analytical tools to precisely identify these low abundance endogenous signaling molecules and accurately measure their behaviorally-relevant concentrations in a complex microenvironment. Our proposed research aims to address this critical knowledge and technological gap by developing new bioanalytical methods to elucidate the complex identities and functional roles of neuropeptides in food intake via combined mass spectrometric and physiological approaches. We employ the crustacean stomatogastric nervous system, cardiovascular system, and its associated neuroendocrine organs as a test-bed for technology development and validation due to the unique advantages and biological significance of this model system. In parallel, we aim to translate our technology development for neuropeptide discovery and analysis to the mammalian central nervous system. To this end, we propose to focus on key brain regions in a rat model at progressively more complex levels of feeding-related information processing. The specific aims include: (1) Developing and applying mass defect-based, amine reactive chemical tags coupled with data-independent acquisition (DIA) mass spectrometry (MS)-based strategy for multiplexed quantitation of neuropeptide changes under different feeding conditions; (2) Developing a nanosecond photochemical reaction (nsPCR)-assisted MALDI-based mass spectral imaging (MSI) technique for mapping co-localization patterns of individual isoforms of extended peptide families and amine neurotransmitters in identified neurons and the feeding circuits, with enhanced sensitivity and chemical information; and (3) Assessing functional roles of neuropeptides in feeding and cardiac regulation using a multi-pronged approach integrating in vivo microdialysis and ex vivo physiological and behavioral measurements. Novel neuropeptides will be evaluated for functional roles in feeding regulation at the neuronal network and system levels. The outcome of the proposed research will be a suite of new analytical tools enabling quantitative assessment of the interplay of neuropeptides and biogenic amines with high spatial, chemical and temporal information. The parallel application of these new methods to both crustacean and mammalian nervous systems in feeding will accelerate our pace towards the development of new therapeutics for feeding disorders.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $431,571 )
2025	2025	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	18	2/20/2025	NON-COMPETING CONTINUATION	$388,413
2025	2025	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	18	6/12/2025	NON-COMPETING CONTINUATION	$43,158
														Subtotal = $431,571

Issue Date FY: 2024 ( Subtotal = $414,308 )
2024	2024	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	17	3/4/2024	NON-COMPETING CONTINUATION	$388,413
2024	2024	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	17	5/3/2024	NON-COMPETING CONTINUATION	$25,895
														Subtotal = $414,308

Issue Date FY: 2023 ( Subtotal = $431,571 )
2023	2023	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	16	4/28/2023	NON-COMPETING CONTINUATION	$431,571
														Subtotal = $431,571

Issue Date FY: 2022 ( Subtotal = $431,571 )
2022	2022	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	15	3/9/2022	COMPETING CONTINUATION	$431,571
2022	2020	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	002	14	7/22/2022	NON-COMPETING CONTINUATION	$0
2022	2020	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6301	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	14	3/2/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $431,571

Issue Date FY: 2020 ( Subtotal = $404,437 )
2020	2020	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	14	5/18/2020	NON-COMPETING CONTINUATION	$404,437
														Subtotal = $404,437

Issue Date FY: 2019 ( Subtotal = $404,437 )
2019	2019	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	13	5/17/2019	NON-COMPETING CONTINUATION	$404,437
														Subtotal = $404,437

Issue Date FY: 2018 ( Subtotal = $404,437 )
2018	2018	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	002	12	5/21/2018	NON-COMPETING CONTINUATION	$404,437
2018	2017	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	11	11/20/2017	COMPETING CONTINUATION	$32,599
2018	2016	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	10	11/20/2017	EXTENSION WITH OR WITHOUT FUNDS	-$32,599
														Subtotal = $404,437

Issue Date FY: 2017 ( Subtotal = $446,941 )
2017	2017	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	11	2/22/2017	COMPETING CONTINUATION	$446,941
2017	2015	UNIVERSITY OF WISCONSIN SYSTEM	21 N PARK ST STE 6401	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	9	11/18/2016	NON-COMPETING CONTINUATION	$0
														Subtotal = $446,941

Issue Date FY: 2016 ( Subtotal = $317,448 )
2016	2016	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	10	6/6/2016	EXTENSION WITH OR WITHOUT FUNDS	$317,448
														Subtotal = $317,448

Issue Date FY: 2015 ( Subtotal = $317,735 )
2015	2015	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	9	5/11/2015	NON-COMPETING CONTINUATION	$317,735
														Subtotal = $317,735

Issue Date FY: 2014 ( Subtotal = $318,015 )
2014	2014	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	8	5/26/2014	NON-COMPETING CONTINUATION	$318,015
														Subtotal = $318,015

Issue Date FY: 2013 ( Subtotal = $307,147 )
2013	2013	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	7	5/20/2013	NON-COMPETING CONTINUATION	$307,147
														Subtotal = $307,147

Issue Date FY: 2012 ( Subtotal = $315,563 )
2012	2012	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	6	12/14/2011	COMPETING CONTINUATION	$315,563
														Subtotal = $315,563

Issue Date FY: 2011 ( Subtotal = $0 )
2011	2010	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	6/27/2011	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2010 ( Subtotal = $275,354 )
2010	2010	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	5	4/21/2010	NON-COMPETING CONTINUATION	$249,354
2010	2010	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Trans-NIH Recovery Act Research Support	000	4	2/8/2010	SUPPLEMENT FOR EXPANSION	$26,000
														Subtotal = $275,354

Issue Date FY: 2009 ( Subtotal = $293,092 )
2009	2009	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	4/22/2009	NON-COMPETING CONTINUATION	$291,607
2009	2009	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 N PARK ST	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	4	9/30/2009	SUPPLEMENT FOR EXPANSION	$1,485
														Subtotal = $293,092

Issue Date FY: 2008 ( Subtotal = $274,594 )
2008	2008	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	4/8/2008	NON-COMPETING CONTINUATION	$235,495
2008	2008	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	3	8/26/2008	SUPPLEMENT FOR EXPANSION	$39,099
														Subtotal = $274,594

Issue Date FY: 2007 ( Subtotal = $240,302 )
2007	2007	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	2	8/14/2007	SUPPLEMENT FOR EXPANSION	$16,000
2007	2007	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	21 NORTH PARK STREET	MADISON	WI	53715	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	2	5/4/2007	NON-COMPETING CONTINUATION	$224,302
														Subtotal = $240,302

Issue Date FY: 2006 ( Subtotal = $254,125 )
2006	2006	UNIVERSITY OF WISCONSIN SYSTEM/BOARD OF REGENTS	750 UNIVERSITY AVENUE	MADISON	WI	53706	DANE	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	4/24/2006	NEW	$254,125
														Subtotal = $254,125

Grand Total All Awards = $6,282,648

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Mass Spectrometric Studies of Neuropeptides in Feeding

Award Number: R01DK071801

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2006

PERIOD OF PERFORMANCE END DATE: 02/28/2027

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