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PNEUMOCOCCAL BIOFILMS IN OTITIS MEDIA

Award Number: R01DC005659
ORGANIZATION: NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2003
PERIOD OF PERFORMANCE END DATE: 02/28/2008

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PNEUMOCOCCAL BIOFILMS IN OTITIS MEDIA - DESCRIPTION (provided by applicant): Otitis media (OM) is the most common reason that an ill child sees a health care provider, receives an antimicrobial or undergoes a general anesthetic. The overprescribing of antibiotics to treat OM is one of the major causes of antibiotic-resistant bacteria. Previous work from the Center for Genomic Sciences (CGS) has shown that culturally-negative middle-ear effusions actually contain bacterial DNA, mRNA and protein. These findings led to the rejection of the previous dogma, which held that chronic OM was a non-bacterial, inflammatory process, with the paradigm that OM is a mucosal biofilm disease. In conjunction with the Center for Biofilm Engineering, CGS has demonstrated that Hemophilus influenza can form biofilms in an animal model and in pediatric otorrhea. Biofilms are complex organizaton of bacteria covered with a protective exopolysaccharide matrix. In this state, bacteria are slow-growing and very resistant to antibiotics. In this application, we extend our work in biofilms by investigating Streptococcus pneumoniae, one of the most common bacteria associated with OM, and the most common respiratory pathogen. Our hypothesis is that understanding the differences in biofilm and planktonic gene expression will identify proteins that can serve either as immunogens for vaccine development, or targets for novel antimicrobial agents. Using state-of- the-art microarray technologies and a robust animal model of OM, the chinchilla, we propose to identify those genes that are necessary for S. pneumoniae to form a biofilm. Combining advances in imaging and molecular biology, we will directly examine the role of the differentially-regulated genes identified by the array technology. Delineating the molecular basis of biofilms will not only advance our understanding of OM, but of chronic infectious disease in general.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2007 ( Subtotal = $564,087 )
2007	2007	ALLEGHENY SINGER RESEARCH CORP	2 ALLEGHENY CTR, 11TH FLOOR	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	5	2/26/2007	NON-COMPETING CONTINUATION	$564,087
														Subtotal = $564,087

Issue Date FY: 2006 ( Subtotal = $568,797 )
2006	2006	ALLEGHENY SINGER RESEARCH CORP	2 ALLEGHENY CTR, 11TH FLOOR	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	4	2/17/2006	NON-COMPETING CONTINUATION	$568,797
														Subtotal = $568,797

Issue Date FY: 2005 ( Subtotal = $678,843 )
2005	2005	ALLEGHENY SINGER RESEARCH CORP	503 MARTINDALE STREET	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	3	3/18/2005	NON-COMPETING CONTINUATION	$678,843
														Subtotal = $678,843

Issue Date FY: 2004 ( Subtotal = $661,072 )
2004	2004	ALLEGHENY SINGER RESEARCH CORP	503 MARTINDALE STREET	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	001	2	3/31/2004	SUPPLEMENT FOR EXPANSION	$110,669
2004	2004	ALLEGHENY SINGER RESEARCH CORP	503 MARTINDALE STREET	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	2	3/19/2004	NON-COMPETING CONTINUATION	$550,403
														Subtotal = $661,072

Issue Date FY: 2003 ( Subtotal = $586,221 )
2003	2003	ALLEGHENY SINGER RESEARCH CORP	503 MARTINDALE STREET	PITTSBURGH	PA	15212	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	1		NEW	$586,221
														Subtotal = $586,221

Grand Total All Awards = $3,059,020

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PNEUMOCOCCAL BIOFILMS IN OTITIS MEDIA

Award Number: R01DC005659

ORGANIZATION: NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2003

PERIOD OF PERFORMANCE END DATE: 02/28/2008

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