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Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse

Award Number: R01DA054317
ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2021
PERIOD OF PERFORMANCE END DATE: 03/31/2026

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Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse - Abstract: Opiate addiction extorts a tremendous toll on society, but a mechanistic understanding of how repeated exposure to opioids such as heroin ultimately results in compulsive drug-taking and -seeking behavior in some individuals, but not others, is still not known. A longstanding idea is that enduring changes in neural circuit function occur because of drug-induced gene expression changes in certain brain cells. This facilitates subsequent drug-taking and -seeking behaviors in vulnerable individuals. Unfortunately, identifying cell-type specific alterations following drug use (typically performed in established animal models of addiction), is generally a slow and tedious process as changes in gene expression following in vivo drug exposure are typically assayed in series, within heterogeneous brain regions, in an a-priori hypothesis driven fashion (i.e. previous knowledge predicting a specific gene may be involved). This dramatically limits the throughput of data collection and likely complicates the subsequent interpretation as gene expression patterns data are typically captured from thousands to millions of homogenized cells. Given that the nervous system is composed of highly heterogeneous tissue, re-assessing cell type specific gene expression changes in an unbiased manner from 1000's of individual cells is desperately needed. Here, we propose to combine our expertise in order to generate comprehensive datasets aimed at understanding how single-cell gene expression, circuit connectivity, and neural activity patterns are impacted by previous drug-taking behavior. These data will provide a much-needed cellular atlas and resource for the addiction neuroscience community and will likely lead to the identification of many novel cell type, gene expression changes, and ensembles that can be leveraged for future study.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $432,271 )
2025	2025	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	001	5	4/11/2025	NON-COMPETING CONTINUATION	$43,226
2025	2025	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	000	5	3/20/2025	NON-COMPETING CONTINUATION	$389,045
														Subtotal = $432,271

Issue Date FY: 2024 ( Subtotal = $526,824 )
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	001	4	4/26/2024	NON-COMPETING CONTINUATION	$52,682
2024	2024	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	000	4	3/13/2024	NON-COMPETING CONTINUATION	$474,142
														Subtotal = $526,824

Issue Date FY: 2023 ( Subtotal = $558,413 )
2023	2023	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	000	3	3/22/2023	NON-COMPETING CONTINUATION	$558,413
														Subtotal = $558,413

Issue Date FY: 2022 ( Subtotal = $588,807 )
2022	2022	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	000	2	3/23/2022	NON-COMPETING CONTINUATION	$588,807
														Subtotal = $588,807

Issue Date FY: 2021 ( Subtotal = $569,937 )
2021	2021	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Drug Use and Addiction Research Programs	000	1	6/17/2021	NEW	$569,937
														Subtotal = $569,937

Grand Total All Awards = $2,676,252

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Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse

Award Number: R01DA054317

ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2021

PERIOD OF PERFORMANCE END DATE: 03/31/2026

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