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Role of TC-NER in the bypass of transcription blocking lesions

Award Number: R01CA315957
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/11/2026
PERIOD OF PERFORMANCE END DATE: 07/31/2031

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Role of TC-NER in the bypass of transcription blocking lesions - Ultraviolet (UV) radiation creates DNA lesions that block RNA Polymerase II (RNAPII), halting transcription and triggering a stress response involving DNA repair, RNAPII removal, and transcriptional shutdown. The consequences of RNAPII bypassing these lesions are not well understood. Although transcription-coupled nucleotide excision repair (TC-NER) is crucial for resolving such DNA lesions, its precise regulation remains debated. Our preliminary studies have identified the acetyltransferase p300 as a key regulator when RNAPII stalls at blocking lesions. p300 interacts with stalled RNAPII and facilitates its removal from damaged chromatin via a USP7-dependent mechanism necessary for transcriptional shutdown after DNA damage. Remarkably, in p300-deficient cells, RNAPII bypasses transcription-blocking lesions and continues transcribing, producing full-length mRNAs despite DNA damage. This persistent transcription and translation activity, along with increased DNA damage, is detrimental to cells. Furthermore, the ability of RNAPII to bypass lesions in p300- deficient cells depends on the recruitment of TC-NER and the activity of TFIIH. Our proposed research aims to: • a) Understand how the bypass of transcription-blocking lesions by RNAPII disrupts the cellular response to DNA damage caused by UV radiation and affects cellular homeostasis. • b) Determine how cells regulate RNAPII stalling at these lesions to prevent bypass and mitigate harmful transcription and translation following DNA damage. By addressing these objectives, our research will enhance the understanding of how our cells maintain DNA integrity after damage.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $522,368 )
2026	2026	UNIVERSITY OF MIAMI	1320 S DIXIE HWY STE 1200	CORAL GABLES	FL	33146	MIAMI-DADE	USA	Cancer Cause and Prevention Research	000	1	8/11/2026	NEW	$522,368
														Subtotal = $522,368

Grand Total All Awards = $522,368

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Role of TC-NER in the bypass of transcription blocking lesions

Award Number: R01CA315957

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/11/2026

PERIOD OF PERFORMANCE END DATE: 07/31/2031

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