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Ultrahigh-throughput imaging of 3D tumor microenvironment at nanoscale

Award Number: R01CA301488
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2025
PERIOD OF PERFORMANCE END DATE: 03/31/2030

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Ultrahigh-throughput imaging of 3D tumor microenvironment at nanoscale - Project Summary This proposal aims to develop an integrated ultrahigh-throughput 3D volumetric super-resolution imaging system by combining a multiscale fluorescence mesoscope with Magnify Expansion Microscopy. Current imaging systems are largely limited to 2D tissue sections at single-cell resolution, which fails to capture the subcellular interactions critical in cancer research. Our innovative system will allow for rapid, large-area 3D imaging with exceptional detail, enabling the study of interactions between cancer and immune cells in situ. We will employ a novel specimen immobilization approach, advanced fluorescence staining techniques, and a fully automated workflow to enhance reproducibility and throughput. By using a genetically engineered mouse model of lung cancer, we will visualize subcellular changes in immune synapses and identify distinct spatial clustering during tumorigenesis and immune invasion. This project will advance the application of spatial biology in cancer research, offering new insights into immune evasion mechanisms and improving immunotherapy strategies. Our technology promises to revolutionize our understanding of cellular interactions in oncology, providing high- precision, large-scale 3D super-resolution imaging capabilities.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $632,300 )
2025	2025	CARNEGIE MELLON UNIVERSITY	5000 FORBES AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Cancer Biology Research	000	1	3/31/2025	NEW	$632,300
														Subtotal = $632,300

Grand Total All Awards = $632,300

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Ultrahigh-throughput imaging of 3D tumor microenvironment at nanoscale

Award Number: R01CA301488

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2025

PERIOD OF PERFORMANCE END DATE: 03/31/2030

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