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Implications of sequential bloodmeals on arbovirus transmission by mosquitoes

Award Number: R01AI148477
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/12/2020
PERIOD OF PERFORMANCE END DATE: 04/30/2025

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Implications of sequential bloodmeals on arbovirus transmission by mosquitoes - Brackney & Armstrong Abstract: Aedes aegypti is the primary vector for a number of human pathogens, including dengue virus (DENV; Flaviviridae, Flavivirus), Zika virus (ZIKV; Flaviviridae, Flavivirus), chikungunya virus (CHIKV; Togaviridae, Alphavirus) and yellow fever virus (YFV; Flaviviridae, Flavivirus), all of which present a continued threat to human health worldwide. Understanding the endemic and epidemic risk of these arthropod-borne (arbo-) viruses is critical to the success of public health preparedness and intervention. One key entomological parameter informing risk estimates is vector competence (how able a mosquito is to become infected and transmit an arbovirus; VC). Quantifying the competency of local vector populations can help inform the risk that any one pathogen poses to a given community. This is often quantified in the laboratory by exposing populations of local mosquitoes to an infectious bloodmeal and harvesting tissues at set time-points post infection. While informative, this approach often fails to consider the biology and behavior of the vector mosquito. For example, it is known that wild Ae. aegypti mosquitoes will imbibe several bloodmeals over the course of a traditional laboratory-based vector competence study (e.g. bloodmeal every two to three days). To address these shortcomings, we recently began examining the effects that multiple blood feeding episodes have on the competency of Ae. aegypti mosquitoes for ZIKV. Our preliminary findings reveal that providing a second non-infectious bloodmeal to ZIKV infected Ae. aegypti mosquitoes enhances viral escape from the midgut and significantly shortens the duration between mosquito acquisition of ZIKV to transmission. In this application we will examine the effects that multiple bloodfeeding episodes have on arbovirus infection of and transmission by vector mosquitoes. Specifically, we will be 1) testing this phenomenon in other virus-vector pairings, 2) evaluating the role of the midgut basal lamina in mediating the double-feed phenotype and 3) determining if similar processes are mediating the ability of arboviruses to infect ovarian tissue and be transmitted vertically.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $300,927 )
2024	2024	CONNECTICUT AGRICULTURAL EXPERIMENTAL STATION	123 HUNTINGTON ST	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Allergy and Infectious Diseases Research	000	5	4/9/2024	NON-COMPETING CONTINUATION	$300,927
														Subtotal = $300,927

Issue Date FY: 2023 ( Subtotal = $298,272 )
2023	2023	CONNECTICUT AGRICULTURAL EXPERIMENT STATION, THE	123 HUNTINGTON STREET	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Allergy and Infectious Diseases Research	000	4	3/24/2023	NON-COMPETING CONTINUATION	$298,272
														Subtotal = $298,272

Issue Date FY: 2022 ( Subtotal = $295,756 )
2022	2022	CONNECTICUT AGRICULTURAL EXPERIMENT STATION, THE	123 HUNTINGTON STREET	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Allergy and Infectious Diseases Research	000	3	3/28/2022	NON-COMPETING CONTINUATION	$295,756
														Subtotal = $295,756

Issue Date FY: 2021 ( Subtotal = $293,370 )
2021	2021	CONNECTICUT AGRICULTURAL EXPERIMENT STATION, THE	123 HUNTINGTON STREET	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Allergy and Infectious Diseases Research	000	2	5/5/2021	NON-COMPETING CONTINUATION	$293,370
														Subtotal = $293,370

Issue Date FY: 2020 ( Subtotal = $293,370 )
2020	2020	CONNECTICUT AGRICULTURAL EXPERIMENT STATION, THE	123 HUNTINGTON STREET	NEW HAVEN	CT	06511	SOUTH CENTRAL CT	USA	Allergy and Infectious Diseases Research	000	1	5/12/2020	NEW	$293,370
														Subtotal = $293,370

Grand Total All Awards = $1,481,695

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Implications of sequential bloodmeals on arbovirus transmission by mosquitoes

Award Number: R01AI148477

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/12/2020

PERIOD OF PERFORMANCE END DATE: 04/30/2025

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