Thursday, November 21, 2024 11/21/2024

Identifying determinants of HIV-1 responsible for the nanoscale distribution and dynamics of virus assembly

Award Number: R01AI138625
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary. Human Immunodeficiency Virus Type-1 (HIV-1) assembly mechanisms, namely the acquisition of the envelope glycoprotein (Env) into the assembling viral lattice, remains inadequately understood. The continued innovation of tools to interrogate these molecular and nanoscale assembly processes is beginning to disclose some of the basic principles. This proposal aims to build upon these innovations and quantify the requirements for Env assembly by interrogating viral and host factor determinants regulating intracellular trafficking, surface display, virus incorporation, and cell-to-cell spread. To accomplish these aims, we will adapt current superresolution and single-molecule tracking approaches developed by our laboratory to understand the three-dimensional structural organization and spatiotemporal dynamics of Env throughout assembly stages and cell-to-cell spread. Next, we will dissect the long Env cytoplasmic tail to understand which residues/regions drive Env incorporation. Finally, we propose to identify the role of host trafficking factors in regulating the surface levels of Env. Our preliminary data supports the feasibility of our approach by demonstrating our ability to extract quantitative information regarding the nanoscale organization and single-molecule dynamics of Env in three- dimensions. These collective approaches will shed new light on HIV-1 assembly and cell-to-cell virus transfer, informing new models and approaches aimed at targeting these processes therapeutically.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $534,168 )
2024	2024	UNIVERSITY OF DENVER	2199 S UNIVERSITY BLVD RM 222	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	6	6/24/2024	COMPETING CONTINUATION	$489,690
2024	2024	UNIVERSITY OF DENVER	2199 S UNIVERSITY BLVD RM 222	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	001	6	9/3/2024	COMPETING CONTINUATION	$44,478
														Subtotal = $534,168

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2022	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	5	8/10/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $369,367 )
2022	2022	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	5	4/26/2022	NON-COMPETING CONTINUATION	$354,458
2022	2022	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	001	5	4/27/2022	SUPPLEMENT FOR EXPANSION	$14,909
														Subtotal = $369,367

Issue Date FY: 2021 ( Subtotal = $416,054 )
2021	2021	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	4	4/12/2021	NON-COMPETING CONTINUATION	$416,054
														Subtotal = $416,054

Issue Date FY: 2020 ( Subtotal = $398,882 )
2020	2020	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	3	3/27/2020	NON-COMPETING CONTINUATION	$354,154
2020	2020	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	001	3	8/7/2020	SUPPLEMENT FOR EXPANSION	$44,728
														Subtotal = $398,882

Issue Date FY: 2019 ( Subtotal = $351,619 )
2019	2019	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	2	4/23/2019	NON-COMPETING CONTINUATION	$351,619
														Subtotal = $351,619

Issue Date FY: 2018 ( Subtotal = $349,084 )
2018	2018	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Allergy and Infectious Diseases Research	000	1	5/9/2018	NEW	$349,084
														Subtotal = $349,084

Grand Total All Awards = $2,419,174

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Identifying determinants of HIV-1 responsible for the nanoscale distribution and dynamics of virus assembly

Award Number: R01AI138625

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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