Wednesday, January 15, 2025
1/15/2025
Project Summary RNA silencing pathways operate in all eukaryotic kingdoms and have been implicated in a range of processes including translational silencing, post-transcriptional RNA degradation, transcriptional gene silencing due to heterochromatin formation or DNA methylation and meiotic elimination or silencing of unpaired DNA. RNA silencing has also been shown to serve as an antivrial defense mechanism in plants, invertebrates, and fungi and to modulate animal virus infections. The antiviral function of RNA silencing not only protects an organism against virus infection, but the generation of virus-derived small RNA products (vsRNAs) and the expression of counter-acting virus-encoded suppressors of RNA silencing can also significantly influence host gene expression and phenotype by impinging on the larger RNA silencing pathway. Although considerable progress has been made in elucidating the core features of the RNA silencing antiviral defense response, and essential components, both host- and virus-encoded, involved in this interaction have been identified, many important details remain unresolved. The Specific Aims of this proposal are designed to address outstanding questions related to a) the origins and properties of vsRNAs, b) the potential for these small RNAs to alter host gene expression in persistent virus infections, and c) molecular mechanisms involved in virus-mediated induction and suppression of RNA silencing pathways. The Research Design takes advantage of a powerful experimental system consisting of a tractable eukaryotic organism, the chestnut blight fungus Cryphonectria parasitica, with a simplified antiviral pathway (involving a single Dicer and single Argonaute) that is the natural host of a constellation of well-characterized viruses with different replication strategies and properties representing five different virus taxonomic families. The results derived from completion of the proposed research will have broad relevance to ongoing efforts to understand the complex interactions between viruses and cellular RNA silencing pathways and the impact that these interactions have on the host. The influence of fungal RNA silencing antiviral pathways clearly needs to be considered in any current and future use of mycoviruses for experimental or therapeutic manipulation of the fungal host, e.g., biological control of fungal virulence or mycotoxin production. Finally, preliminary data already indicates that the evolutionary position of fungi relative to animals and plants will provide insights into novel mechanisms for the induction and suppression of RNA silencing pathways yet to be revealed in the other organisms.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).