Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

INTESTINAL BACTERIA AND RABBIT ANTIBODY DIVERSIFICATION

Award Number: R01AI049458
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: HEALTH SERVICES
PERIOD OF PERFORMANCE START DATE: 09/01/2002
PERIOD OF PERFORMANCE END DATE: 06/30/2007

Group Awards By:

View Award Description

INTESTINAL BACTERIA AND RABBIT ANTIBODY DIVERSIFICATION - DESCRIPTION (provided by applicant): The rabbit primary antibody repertoire is generated through somatic diversification of a limited initial repertoire in which most VDJ gene rearrangements utilize the 3'-most VH gene segment, VH1. The initial repertoire is diversified by somatic hypermutation and a somatic gene conversion-like mechanism in gut-associated lymphoid tissue (GALT) 1 to 2 months after birth. Studies of germ-free rabbits and of rabbits in which the cecum was ligated at birth to prevent entry of intestinal contents into the appendix have shown that GALT development is dependent on an exogenous factor(s) derived from the gut. Our laboratory has extended these observations by showing, in rabbits in which the appendix was ligated at birth and all other organized GALT was removed (ligApx rabbits), that repertoire diversification is also dependent on an exogenous factor(s) derived from the gut. In order to identify this exogenous factor(s), we have hand-raised sterilely derived rabbits on a sterile diet away from contact with conventional rabbits and their microflora (remote colony rabbits) and found that most of these rabbits do not develop normal GALT or diversify their antibody repertoires. Since these remote colony rabbits are not germ-free, these results suggest that specific members of normal intestinal microflora are required for both normal GALT development and repertoire diversification. Using conventional culture methods to examine differences in the intestinal microflora of conventional and remote colony rabbits, we have not been able to identify bacterial species that drive rabbit GALT development and repertoire diversification. Approaches to the study of microbial diversity based on bacterial 16S rDNA have overcome many of the limitations of culture-based methods and made available sensitive techniques for studying intestinal microflora. We propose in this grant application to use one such approach, in combination with detailed temporal analysis of GALT development and VDJ gene diversification, to identify bacterial species that stimulate rabbit GALT development and repertoire diversification, and determine the mechanism by which they influence these developmental processes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2006 ( Subtotal = $216,783 )
2006	2006	LOYOLA UNIVERSITY MEDICAL CENTER	820 N MICHIGAN AVENUE	CHICAGO	IL	60611	COOK	USA	Allergy and Infectious Diseases Research	000	5	6/15/2006	NON-COMPETING CONTINUATION	$216,783
														Subtotal = $216,783

Issue Date FY: 2005 ( Subtotal = $222,000 )
2005	2005	LOYOLA UNIVERSITY MEDICAL CENTER							Allergy and Infectious Diseases Research	000	4	6/16/2005	NON-COMPETING CONTINUATION	$222,000
														Subtotal = $222,000

Issue Date FY: 2004 ( Subtotal = $222,000 )
2004	2004	LOYOLA UNIVERSITY MEDICAL CENTER							Allergy and Infectious Diseases Research	000	3	7/1/2004	NON-COMPETING CONTINUATION	$222,000
														Subtotal = $222,000

Issue Date FY: 2003 ( Subtotal = $222,000 )
2003	2003	LOYOLA UNIVERSITY MEDICAL CENTER	2160 SOUTH FIRST AVENUE	MAYWOOD	IL	60153	COOK	USA	Allergy and Infectious Diseases Research	000	2	5/19/2003	NON-COMPETING CONTINUATION	$222,000
														Subtotal = $222,000

Issue Date FY: 2002 ( Subtotal = $199,800 )
2002	2002	LOYOLA UNIVERSITY MEDICAL CENTER	2160 SOUTH FIRST AVENUE	MAYWOOD	IL	60153	COOK	USA	Allergy and Infectious Diseases Research	000	1	8/19/2002	NEW	$199,800
														Subtotal = $199,800

Grand Total All Awards = $1,082,583

Top

All Categories

About

Search

Reports

Data Submission

Award Information

INTESTINAL BACTERIA AND RABBIT ANTIBODY DIVERSIFICATION

Award Number: R01AI049458

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: HEALTH SERVICES

PERIOD OF PERFORMANCE START DATE: 09/01/2002

PERIOD OF PERFORMANCE END DATE: 06/30/2007

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer