Thursday, January 1, 2026 1/1/2026

Cell Biology of Reovirus Infection

Award Number: R01AI032539
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/1992
PERIOD OF PERFORMANCE END DATE: 12/31/2029

Group Awards By:

View Award Description

Cell Biology of Reovirus Infection - Most viruses that replicate in the cytoplasm form neoorganelles that serve as sites of viral genome replication and particle assembly. These highly specialized viral factory (VF) structures concentrate viral replication proteins and nucleic acids, limit activation of cell-intrinsic defenses, and coordinate release of progeny particles. Despite the importance of VFs in viral replication, there are key gaps in knowledge about how these organelles form and function. Reovirus is a genetically tractable double-stranded RNA (dsRNA) virus that has been linked to celiac disease and shows promise for oncolytic applications. The proposed research will define mechanisms of reovirus factory formation, genome replication and translation, and progeny particle release. In common with other dsRNA viruses, including important pathogens of animals (orbiviruses) and humans (rotaviruses), reovirus factories are nucleated by viral nonstructural proteins that recruit viral structural proteins for genome replication and capsid assembly. We have discovered that (i) nonstructural protein µNS forms VF-like structures that resemble biomolecular condensates formed by liquid-liquid phase-separation (LLPS), (ii) nonstructural protein σNS interacts with RNA polymerase λ3 to promote RNA synthesis, and µNS interacts with host protein ATXN2L to enhance viral translation, and (iii) progeny particles are transported from factories and released using a vesicular sorting mechanism dependent on modified lysosomes and actin-like filaments. Three integrated specific aims are proposed to fill knowledge gaps about reovirus factory biogenesis, replication and translation, and progeny particle egress. In Specific Aim 1, VF properties of µNS will be elucidated using biochemical and cell-imaging assays established to define LLPS condensates. The structure of µNS will be determined using X- ray crystallography and cryo-electron microscopy (cryo-EM). Host proteins that contribute to factory maturation will be examined using rapid proximity-dependent biotin identification (TurboID) of µNS-interacting partners. In Specific Aim 2, mechanisms by which reovirus factories facilitate viral replication and translation will be evaluated. The function of σNS in viral genome replication will be defined by determining the structure of σNS in complex with λ3 and RNA along with assays of RNA polymerase activity. The function of ATXN2L in synthesis of viral proteins will be determined using ribosomal sequencing and cellular and in vitro translation assays. In Specific Aim 3, the process by which mature virions are transported to the cell surface and released will be visualized using single-particle cryo-EM and cryo-electron tomography. Host proteins required for nonlytic reovirus egress will be identified using candidate and unbiased screening approaches and validated using replication and egress assays. These studies will enhance an understanding of mechanisms by which pathogenic viruses alter cellular architecture to engineer replication organelles, coordinate key replication functions, and exit infected cells. This information may foster development of antiviral drugs that impede these essential viral replication steps and improve the use of reovirus as an oncolytic therapeutic.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $679,958 )
2026	2026	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	34	12/29/2025	NON-COMPETING CONTINUATION	$679,958
														Subtotal = $679,958

Issue Date FY: 2025 ( Subtotal = $674,101 )
2025	2025	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	001	33	1/24/2025	COMPETING CONTINUATION	$674,101
2025	2025	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	003	33	6/10/2025	COMPETING CONTINUATION	$27,748
2025	2023	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	32	1/15/2025	NON-COMPETING CONTINUATION	$0
2025	2023	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	002	32	6/10/2025	NON-COMPETING CONTINUATION	-$27,748
														Subtotal = $674,101

Issue Date FY: 2023 ( Subtotal = $509,806 )
2023	2023	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	32	3/27/2023	NON-COMPETING CONTINUATION	$509,806
														Subtotal = $509,806

Issue Date FY: 2022 ( Subtotal = $516,414 )
2022	2022	UNIVERSITY OF PITTSBURGH, THE	4200 5TH AVE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	31	3/22/2022	NON-COMPETING CONTINUATION	$516,414
														Subtotal = $516,414

Issue Date FY: 2021 ( Subtotal = $518,440 )
2021	2021	UNIVERSITY OF PITTSBURGH, THE	4200 5TH AVE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	30	3/5/2021	NON-COMPETING CONTINUATION	$518,440
														Subtotal = $518,440

Issue Date FY: 2020 ( Subtotal = $520,407 )
2020	2020	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	29	3/25/2020	NON-COMPETING CONTINUATION	$520,407
														Subtotal = $520,407

Issue Date FY: 2019 ( Subtotal = $550,292 )
2019	2019	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	002	28	9/26/2019	COMPETING CONTINUATION	$200,842
2019	2019	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	28	4/10/2019	COMPETING CONTINUATION	$550,292
2019	2018	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	001	27	9/26/2019	NON-COMPETING CONTINUATION	-$200,842
														Subtotal = $550,292

Issue Date FY: 2018 ( Subtotal = $466,908 )
2018	2018	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	000	27	3/30/2018	NON-COMPETING CONTINUATION	$466,908
														Subtotal = $466,908

Issue Date FY: 2017 ( Subtotal = $469,215 )
2017	2017	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	004	26	3/17/2017	NON-COMPETING CONTINUATION	$465,448
2017	2016	VANDERBILT UNIVERSITY MEDICAL CENTER	1161 21ST AVE S STE D3300 MCN	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	24	11/25/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$421,361
2017	2016	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	003	25	2/24/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$3,310
2017	2016	UNIVERSITY OF PITTSBURGH THE	3520 FIFTH AVE	PITTSBURGH	PA	15213	ALLEGHENY	USA	Allergy and Infectious Diseases Research	001	25	11/25/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$425,128
2017	2016	VANDERBILT UNIVERSITY MEDICAL CENTER	1161 21ST AVE S STE D3300 MCN	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	002	24	2/24/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$3,310
														Subtotal = $469,215

Issue Date FY: 2016 ( Subtotal = $480,027 )
2016	2016	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	001	23	5/17/2016	NON-COMPETING CONTINUATION	-$463,407
2016	2016	VANDERBILT UNIVERSITY MEDICAL CENTER	1161 21ST AVE S STE D3300 MCN	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	002	24	5/17/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$465,154
2016	2016	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	23	3/28/2016	NON-COMPETING CONTINUATION	$478,280
														Subtotal = $480,027

Issue Date FY: 2015 ( Subtotal = $478,280 )
2015	2015	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	22	3/12/2015	NON-COMPETING CONTINUATION	$478,280
														Subtotal = $478,280

Issue Date FY: 2014 ( Subtotal = $506,214 )
2014	2014	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	001	21	3/17/2014	COMPETING CONTINUATION	$506,214
2014	2013	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	20	3/14/2014	NON-COMPETING CONTINUATION	$0
														Subtotal = $506,214

Issue Date FY: 2013 ( Subtotal = $460,616 )
2013	2013	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	20	2/28/2013	NON-COMPETING CONTINUATION	$441,015
2013	2013	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	002	20	3/14/2014	NON-COMPETING CONTINUATION	$0
2013	2013	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	001	20	6/4/2013	NON-COMPETING CONTINUATION	$19,601
														Subtotal = $460,616

Issue Date FY: 2012 ( Subtotal = $490,017 )
2012	2012	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	19	2/22/2012	NON-COMPETING CONTINUATION	$490,017
														Subtotal = $490,017

Issue Date FY: 2011 ( Subtotal = $489,623 )
2011	2011	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	001	18	8/8/2011	NON-COMPETING CONTINUATION	$33,280
2011	2011	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	18	2/11/2011	NON-COMPETING CONTINUATION	$456,343
														Subtotal = $489,623

Issue Date FY: 2010 ( Subtotal = $492,628 )
2010	2010	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	17	2/19/2010	NON-COMPETING CONTINUATION	$492,628
														Subtotal = $492,628

Issue Date FY: 2009 ( Subtotal = $499,272 )
2009	2009	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	001	16	2/27/2009	COMPETING CONTINUATION	$499,272
2009	2007	VANDERBILT UNIVERSITY HOSPITAL, CHILDREN'S REHAB UNIT	1161 21ST AVENUE SOUTH	NASHVILLE	TN	37232	DAVIDSON	USA	Allergy and Infectious Diseases Research	000	15	2/27/2009	NON-COMPETING CONTINUATION	$0
														Subtotal = $499,272

Grand Total All Awards = $12,803,377

Page 1 of 2 (70 items)12

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Cell Biology of Reovirus Infection

Award Number: R01AI032539

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/1992

PERIOD OF PERFORMANCE END DATE: 12/31/2029

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer