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Assessment of murine retinal acuity ex vivo by machine learning of multielectrode array recordings

Award Number: R00EY031333
ORGANIZATION: NATIONAL EYE INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2020
PERIOD OF PERFORMANCE END DATE: 04/30/2027

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Assessment of murine retinal acuity ex vivo by machine learning of multielectrode array recordings - Project Summary: Darwin Babino, PhD, a trained pharmacologist/electrophysiologist, has spent the last ten years working on several disciplines in the vision sciences. His proposal entitled “Assessment of murine retinal acuity ex vivo by machine learning of multielectrode array recordings” presents his overarching goal to improve vision restoration approaches by developing methods to test the potential of these techniques thereby accelerating the development of effective interventions. Dr. Babino and his primary mentor, Dr. Russell Van Gelder, have assembled a strong team of co-mentors at the University of Washington SOM and collaborators to guide him through the proposed training and research. His previous training will be supplemented with goals to help his development as an independent investigator: 1) Study design and practical learning in performing panretinal (MEA) biological experiments; 2) Fundamental and advanced techniques of the proposed optogenetic and stem-cell restoration techniques; 3) Application of advanced machine learning techniques; 4) Develop leadership and professional skills to establish an independent group. The ability to assess the function of panretinal circuitry will foster our understanding of the advantages and weaknesses of different restoration techniques (Aim 1). The work proposed here will improve an existing retinal acuity assessment tool which combines machine learning techniques on novel, high-density multielectrode array recordings of ganglion cell responses in several mouse models. The utility of this system will be demonstrated in assessing visual potential of the mouse retina in three different approaches to vision restoration that are challenging for in vivo assessment (Aim 2). In collaboration with Dr. Deepak A. Lamba at UCSF, we will apply our system to animals which have undergone stem-cell replacement of retinal cells including photoreceptor cells. An optogenetics approach will also be evaluated in collaboration with Dr. John Flannery at UC Berkeley whose group has developed vectors for expressing rhodopsin and cone opsins in ganglion and bipolar cells. Finally, differences between native and restored vison with small molecule photoswitches, light-activated inhibitors of voltage-gated potassium channels, which confer light-dependent firing on treated cells, will be assessed. The resulting advanced electrophysiology application will help elucidate fundamental questions about the functional retina, mechanisms that lead to retinal degeneration and the potential of several therapeutics for the treatment of retinal diseases. Furthermore, this career development award will facilitate Dr. Babino’s development into an independent investigator by priming an R01 grant application.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $248,391 )
2026	2026	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	5	6/25/2026	NON-COMPETING CONTINUATION	$248,391
														Subtotal = $248,391

Issue Date FY: 2025 ( Subtotal = $248,744 )
2025	2025	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	4	6/2/2025	NON-COMPETING CONTINUATION	$248,744
														Subtotal = $248,744

Issue Date FY: 2024 ( Subtotal = $249,000 )
2024	2024	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	3	6/13/2024	EXTENSION WITH OR WITHOUT FUNDS	$0
2024	2024	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	3	4/17/2024	EXTENSION WITH OR WITHOUT FUNDS	$249,000
														Subtotal = $249,000

Grand Total All Awards = $746,135

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Assessment of murine retinal acuity ex vivo by machine learning of multielectrode array recordings

Award Number: R00EY031333

ORGANIZATION: NATIONAL EYE INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2020

PERIOD OF PERFORMANCE END DATE: 04/30/2027

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