National Native HIV Network Technical Assistance Center - Addressing persistent disparities in HIV prevention and treatment among American Indian/Alaska Native (AI/AN) communities is crucial in the United States. Despite significant strides in HIV awareness, AI/AN populations continue to face disproportionate burdens of HIV incidence and prevalence. In 2021, 223 new HIV diagnoses were reported among AI/AN individuals, underlining the urgent need for targeted interventions. Compounded by high rates of sexually transmitted infections (STIs) and Hepatitis C, these disparities demand immediate action. To tackle these challenges, the Albuquerque Area Indian Health Board, Inc. (AAIHB) proposes the National Native HIV Network Technical Assistance Center. This initiative aims to enhance the capacity of AI/AN communities to implement evidence-based HIV prevention and treatment strategies. By providing culturally appropriate technical assistance, training, and support to CDC-funded organizations, tribal health departments, and community-based organizations, the project seeks to empower the HIV workforce to plan, implement, and evaluate comprehensive prevention programs. Over a 5-year period, the National Native HIV Network Technical Assistance Center endeavors to cultivate a highly skilled HIV workforce focused on reducing new infections, increasing access to care, and improving health outcomes for AI/AN individuals. Short-term objectives include strengthening organizational capacity and structural environments to support effective HIV prevention programs. Intermediate-term goals prioritize addressing syndemic conditions and social determinants of health (SDOH) that contribute to HIV-related health disparities among AI/AN people. Through tailored strategies and activities, the NNHN TAC will deliver capacity-building assistance to a diverse workforce, including professional, technical, clinical, and managerial staff within CDC-funded organizations and tribal health departments. Training and support will address challenges related to HIV prevention, reducing disparities, and promoting health equity. Continuous monitoring, evaluation, and collaboration with partners will ensure progress toward achieving project goals and fostering sustainable improvements in HIV prevention and treatment outcomes within AI/AN communities.