The Portland National HIV Behavioral Surveillance (NHBS) is a bio-behavioral surveillance project conducted among three rotating populations overburdened by HIV acquisition and transmission: low-income heterosexuals; gay, bisexual, and other men who have sex with men (MSM); and, people who inject drugs (PWID). Portland NHBS engages local public health, community, and academic partners in collecting, analyzing, and disseminating timely data on key demographics, substance use, sexual behavior, social determinants of health, health inequities, and access to and use of biomedical and behavioral HIV prevention and care. Portland NHBS works with partners to put these data into action to inform the implementation of key strategies to end the HIV epidemic in the Portland metropolitan statistical area. These strategies include: increasing uptake of HIV testing and knowledge of serostatus; increasing knowledge of, access to, and use of pre-exposure prophylaxis; increasing access to and use of critical harm reduction services; increasing access to and engagement in HIV treatment; and, guiding HIV cluster detection and response. Underpinning all these strategies is the goal of HIV health equity. Portland NHBS strives to prioritize people historically marginalized by public health systems, including women, people who use drugs, and those who identify as Black, Latinx, indigenous, and gender diverse.