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Breadth and mechanism of Wolbachia-based inhibition of fungal infection in Drosophila

Award Number: K99AI180425
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP
PERIOD OF PERFORMANCE START DATE: 07/01/2024
PERIOD OF PERFORMANCE END DATE: 08/24/2025

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Breadth and mechanism of Wolbachia-based inhibition of fungal infection in Drosophila - Project Summary: Host-symbiont interactions represent an enormous variety of relationships with an extraordinary array of resulting phenotypes. Microbial symbionts of arthropods have a particularly wide range of symbiotic interactions with their hosts. One exceptional case is that of Wolbachia bacterial symbionts that are found in almost half of all arthropod species. These microbes are genetically and phenotypically highly diverse, and various strains have abilities ranging from manipulation of host reproduction to provision of essential host nutrients. One unique and highly consequential feature of some Wolbachia strains is the ability to inhibit transmission of viruses including Zika and dengue from arthropods to humans. Several global initiatives have taken advantage of this ability to use Wolbachia to successfully fight arthropod-borne disease. However, the mechanism of pathogen- blocking is unclear, as is the breadth of the phenotype in terms of affected pathogens. This limits the ability to predict the success of current vector control field trials and to identify and develop new applications of the phenotype. Preliminary data shows that Wolbachia pathogen inhibition extends to diverse fungi as well, since Wolbachia-positive Drosophila melanogaster flies live longer after systemic infection with a variety of fungal pathogens. It also suggests host immune priming may underlie the mechanism. This proposal outlines a set of organismal, genetic, and molecular approaches to build a comprehensive profile of Wolbachia-fungal interactions in Dipteran hosts. This proposal will primarily use the elite genetic model, Drosophila melanogaster, with the wMel Wolbachia strain, the same strain that is utilized in antiviral initiatives. Aim 1 will functionally assess the role of host immune priming in the mechanism, focusing on specific genes of interest in the Toll and JAK/STAT pathways, using genetic and transcriptomic approaches. In Aim 2, trans-infections, feeding assays, and metabolomics will be used to assess the role of Wolbachia factors, nutritional competition, and fungal metabolite inhibition in the phenotype. Aim 3 will test translational potential by assessing fungal protection in Aedes mosquitoes, inhibition of inter-individual fungal transmission, and the population benefits of fungal protection in cage populations. This approach leverages many powerful approaches and techniques to provide an extensive characterization of Wolbachia-fungal interactions. Doing so will provide insight into Wolbachia pathogen blocking mechanisms, the basic biology of interkingdom microbial interactions, and potentially inform and bolster vector control initiatives. With guidance from Advisory Committee members Drs. Ackley, Oakley, and Michel and sponsor Dr. Unckless at the University of Kansas, this proposal encompasses a comprehensive plan with all of the training needed for Dr. Perlmutter to achieve the career goal of becoming an independent investigator studying the genetics and mechanisms of host-symbiont interactions with a main focus on arthropods.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = -$75,206 )
2026	2025	UNIVERSITY OF KANSAS CENTER FOR RESEARCH INC	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Allergy and Infectious Diseases Research	000	2	12/30/2025	NON-COMPETING CONTINUATION	-$75,206
2026	2025	UNIVERSITY OF KANSAS CENTER FOR RESEARCH INC	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Allergy and Infectious Diseases Research	001	2	3/2/2026	NON-COMPETING CONTINUATION	$0
														Subtotal = -$75,206

Issue Date FY: 2025 ( Subtotal = $120,209 )
2025	2025	UNIVERSITY OF KANSAS CENTER FOR RESEARCH INC	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Allergy and Infectious Diseases Research	000	2	7/15/2025	NON-COMPETING CONTINUATION	$120,209
														Subtotal = $120,209

Issue Date FY: 2024 ( Subtotal = $122,634 )
2024	2024	UNIVERSITY OF KANSAS CENTER FOR RESEARCH INC	2385 IRVING HILL RD	LAWRENCE	KS	66045	DOUGLAS	USA	Allergy and Infectious Diseases Research	000	1	6/28/2024	NEW	$122,634
														Subtotal = $122,634

Grand Total All Awards = $167,637

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Breadth and mechanism of Wolbachia-based inhibition of fungal infection in Drosophila

Award Number: K99AI180425

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: TRAINING/TRAINEESHIP

PERIOD OF PERFORMANCE START DATE: 07/01/2024

PERIOD OF PERFORMANCE END DATE: 08/24/2025

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