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Integrating highly multiplexed immunofluorescence with label-free 2D hyperspectral chemical imaging to interrogate the colorectal cancer tumor-invasive front

Award Number: F32CA294924
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 07/15/2024
PERIOD OF PERFORMANCE END DATE: 07/14/2027

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Integrating highly multiplexed immunofluorescence with label-free 2D hyperspectral chemical imaging to interrogate the colorectal cancer tumor-invasive front - Project Summary/Abstract Colorectal cancer (CRC) is the second most common cause of cancer-related death in the United States. Despite advances in both diagnostic and therapy, a large inter-individual variability in therapy response and diagnostic accuracy can be observed. The optimization of medical imaging agents in CRC is a critical component for improving our technical capabilities relating to early diagnostics, complete labeling of tumor-positive tissue for surgical resection, surveillance, and precision therapy. One major requirement for improving the clinical performance of therapeutics and medical imaging agents is the characterization of structural and functional chemistries in cancerous tissue that are either different from or absent in normal tissue. This is especially important in regions that may not be sufficiently labeled by existing imaging agents with a clinically acceptable and implementable signal-to-background ratio. In these cases, curative surgical intervention achieves mixed success in the CRC patient population due to the technical inability to label accurately and precisely tumor- positive tissues. Moreover, these differences may potentially explain the clinically observed variation in patient therapy response and disease aggressiveness. To develop a computational workflow that integrates CODEX imaging with high-dimensional hyperspectral chemical mapping and test my hypothesis, my experimental structure is divided into two main components. (1) I will perform preliminary non-destructive, label-free spatiochemical imaging on normal and CRC patient biopsies to identify differences in the functional heterogenous chemistries. (2) I will perform spatial omic identification of CRC related tissue architectures using CODEX for integration with the high-dimensional label-free 2D infrared spectral maps. The innovation of this research is the development of the computational workflow and framework that integrates highly multiplexed CODEX with high-dimensional label-free 2D hyperspectral chemical imaging. This approach can lead an alternative representation of physicochemical tissue properties for the characterization and identification of spatiochemistries relevant to CRC and its tumor-invasive front. Long-term, this can be used in the future to guide the development and optimization of cancer imaging agents to improve patient overall outcomes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $81,580 )
2026	2026	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Cancer Research Manpower	000	3	6/10/2026	NON-COMPETING CONTINUATION	$81,580
														Subtotal = $81,580

Issue Date FY: 2025 ( Subtotal = $78,040 )
2025	2025	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Cancer Research Manpower	000	2	7/15/2025	NON-COMPETING CONTINUATION	$78,040
														Subtotal = $78,040

Issue Date FY: 2024 ( Subtotal = $74,284 )
2024	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Cancer Research Manpower	001	1	6/17/2024	NEW	$0
2024	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Cancer Research Manpower	000	1	6/17/2024	NEW	$74,284
														Subtotal = $74,284

Grand Total All Awards = $233,904

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Integrating highly multiplexed immunofluorescence with label-free 2D hyperspectral chemical imaging to interrogate the colorectal cancer tumor-invasive front

Award Number: F32CA294924

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 07/15/2024

PERIOD OF PERFORMANCE END DATE: 07/14/2027

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