Thursday, November 21, 2024
11/21/2024
DESCRIPTION (provided by applicant): Facioscapulohumeral muscular dystrophy (FSHD) is a rare disease affecting 1;15,0000-20,000 individuals and is clinically characterized by progressive weakness and wasting of the facial and upper extremity muscles. In FSHD patients, due to the contraction of the D4Z4 macrosatellite repeat array on chromosome 4 (FSHD1), or due to SMCHD1 mutations (FSHD2), the somatic repression of the retrogene DUX4, which encodes the germline double homeobox 4 transcription factor, is incomplete leading to the variegated expression of the germline transcription factor in muscle fibers. SMCHD1 encodes a protein that regulates chromatin repression at different loci, and is necessary for the establishment and maintenance of the monoallelic expression of the protocadherin (PCDH) gene cluster on human chromosome 5. Preliminary studies show that PCDH gene cluster regulation is impaired in SMCHD1 mutation carriers. The objectives of this project are to (1) determine the exact role of SMCHD1 in the regulation of the PCDH gene cluster in neuronal and non-neuronal (muscle) cells, to (2) document the extend of PCDH gene cluster dysregulation in SMCHD1 mutation carriers and to (3) determine the functional consequence of PCDH gene cluster dysregulation in muscle cells. To accomplish these objectives I will combine quantitative chromosome immunoprecipitation followed by sequencing (ChIP- seq) and ChIP-PCR experiments to interrogate the regulation of the PCDH gene cluster in FSHD2 patient primary muscle cells and, as a reference, in neuronal SK-N-SH cells. This I will combine with functional genomics strategies (knock down and overexpression of critical regulators of the PCDH gene cluster) to dissect the function and hierarchy of these chromatin modifiers in PCDH gene cluster regulation, and functional studies of muscle cell differentiation to establish the role of PCDH gene cluster proteins in a non-neuronal context. The long term goal of these lines of investigation is to uncover the consequences of FSHD2 mutations on PCDH gene cluster regulation and to understand how these alterations in transcription are leading to FSHD phenotypes.
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