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Designing addressable proteoliposomes by using viral transmembrane proteins and biomimetic cell-free synthesis techniques.

Award Number: F32AI183691
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 05/01/2024
PERIOD OF PERFORMANCE END DATE: 04/30/2027

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Designing addressable proteoliposomes by using viral transmembrane proteins and biomimetic cell-free synthesis techniques. - Project Summary /Abstract Viruses present one of the most efficient mechanisms for intracellular cargo (i.e. viral genome) delivery in which interactions at the virus-host cell interface dictate the delivery pathway. For instance, enveloped viruses- those that are 'wrapped' in a lipid bilayer, deliver their genetic cargo by first interacting with extracellular receptors, triggering a reaction cascade that results in fusion of the virus- and host cell lipid membranes and cargo release into the cytosol. Harnessing the efficiency of this translocation mechanism would drastically improve cellular uptake of therapeutic and bioactive cargo, currently a major obstacle in both agricultural and pharmaceutical communities, each with major impact on human health. The research proposed in this fellowship aims to repurpose viral fusion machinery for delivering user-defined cargo to cells containing the appropriate receptors. More specifically, several virus-derived proteins have been chosen including Hemagglutinin (HA) - from Influenza, glycoprotein G (NiV-G) and fusion protein F (NiV- F) - from Nipah virus, and Spike protein - from SARS-CoV-2. These proteins represent a small selection of model proteins, all of which interact with different receptor types found on the cellular surface, providing a potential handle for targeting cells that abundantly display the specific receptors. To circumvent challenges associated with using infectious viruses or isolating membrane proteins, we will concurrently adapt existing cell-free synthesis (CFPS) techniques to produce membrane proteins and efficiently insert them into our delivery vehicles of choice- liposomes. The short-term goals of this project are to demonstrate 1) virus fusion-protein activity and delivery, and 2) improved efficiency of virus membrane insertion into liposomes using the adapted CFPS methodologies. The long-term goals include tuning the biodistribution capabilities, afforded by the virus-derived proteins, to deliver cargo to discrete and specific locations within the human body or other organism. This fellowship will provide the applicant with the financial support needed to design and test the proteoliposome-based delivery system and develop ideas that will aid the applicant's independent research program in the field of virus-inspired biomaterials.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $81,580 )
2026	2026	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	3	4/27/2026	NON-COMPETING CONTINUATION	$81,580
														Subtotal = $81,580

Issue Date FY: 2025 ( Subtotal = $78,040 )
2025	2025	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	2	8/21/2025	NON-COMPETING CONTINUATION	$78,040
														Subtotal = $78,040

Issue Date FY: 2024 ( Subtotal = $74,284 )
2024	2024	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	002	1	5/9/2024	NEW	$4,784
2024	2024	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	001	1	3/13/2024	NEW	$0
2024	2024	CORNELL UNIVERSITY	341 PINE TREE RD	ITHACA	NY	14850	TOMPKINS	USA	Allergy and Infectious Diseases Research	000	1	3/13/2024	NEW	$69,500
														Subtotal = $74,284

Grand Total All Awards = $233,904

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Designing addressable proteoliposomes by using viral transmembrane proteins and biomimetic cell-free synthesis techniques.

Award Number: F32AI183691

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 05/01/2024

PERIOD OF PERFORMANCE END DATE: 04/30/2027

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