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AAV gene therapy to restore fertility in mammalian Sertoli cell dysfunction models

Award Number: F31HD114406
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 03/01/2024
PERIOD OF PERFORMANCE END DATE: 02/28/2027

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AAV gene therapy to restore fertility in mammalian Sertoli cell dysfunction models - ABSTRACT: Infertility impacts 48 million couples worldwide and 50% of cases are due to a male factor. Non-obstructive azoospermia (NOA) is the absence of sperm due to spermatogenic failure. Some cases are due to known conditions (Klinefelter’s Syndrome, Y chromosome microdeletions, and hypogonadism) but many cases are idiopathic (unexplained). Recent identification of novel monogenic causes of NOA sparks the possibility of gene therapy as a potential treatment option. There are ethical, legal and societal concerns about gene therapy approaches that would modify the germline and potentially be passed to progeny. Therefore, development of gene therapy approaches for male infertility should consider the risk of germline modification and transmission. Sertoli cells are the only testicular somatic cells that are inside the seminiferous tubules and in direct contact with the germ cells and Sertoli cell mutations can lead to NOA in both mice and humans. I will test the feasibility and safety of in vivo Sertoli cell gene therapy in mouse models of human NOA. Adeno-associated virus (AAV) is an optimal vector for Sertoli cell gene therapy because there are many AAV serotypes with cell type specificity of infection, it can have long lasting expression, it has low toxicity in the testis, and does not typically integrate into the genome. AAV has been used to introduce a corrective Kitl gene into Sertoli cells of Kitl-/- mice that have an NOA phenotype, restoring spermatogenesis. In humans, mutations in the KITL gene do not cause infertility. Under separate funding, our group has been performing whole genome or whole exome sequencing to identify genetic variants in men with NOA. I have screened those data to identify NOA-associated variants in MAP7 and CLCN2 that are expressed by Sertoli cells and cause NOA when knocked out in mice. I will use CRISPR/Cas9 gene editing to introduce the patient variants into the orthologous regions of the mouse genome to create new mouse models of human NOA. I will use these models to test the hypothesis that, fertility can be safely restored by AAV-mediated Sertoli cell gene therapy and that this can be accomplished without germline modification or transmission to progeny. To facilitate the translation of this technology toward the human clinic, I will utilize a novel human testis organ culture technique to identify AAV serotypes that exhibit Sertoli cell specific infection in human testicular tissue. Completion of my project will demonstrate the safety and feasibility of Sertoli cell gene therapy using AAV vectors. I will uncover new knowledge into genetic causes of Sertoli cell dysfunction and propose a technique that can test other aspects of AAV use in human tissue. Overall, the completion of this grant will greatly increase my technical research skills in gene therapy and male infertility in a bench to bedside research pipeline. This will provide a strong foundation of knowledge and skills to prepare me for a career as a faculty and independent researcher in academia.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $50,114 )
2026	2026	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	000	3	3/9/2026	NON-COMPETING CONTINUATION	$49,538
2026	2026	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	001	3	3/24/2026	NON-COMPETING CONTINUATION	$576
														Subtotal = $50,114

Issue Date FY: 2025 ( Subtotal = $49,538 )
2025	2025	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	001	2	7/24/2025	NON-COMPETING CONTINUATION	$564
2025	2025	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	000	2	2/20/2025	NON-COMPETING CONTINUATION	$48,974
														Subtotal = $49,538

Issue Date FY: 2024 ( Subtotal = $47,694 )
2024	2024	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	001	1	2/12/2024	NEW	$0
2024	2024	MAGEE-WOMENS RESEARCH INSTITUTE AND FOUNDATION	3240 CRAFT PL STE 100	PITTSBURGH	PA	15213	ALLEGHENY	USA	Child Health and Human Development Extramural Research	000	1	2/12/2024	NEW	$47,694
														Subtotal = $47,694

Grand Total All Awards = $147,346

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AAV gene therapy to restore fertility in mammalian Sertoli cell dysfunction models

Award Number: F31HD114406

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 03/01/2024

PERIOD OF PERFORMANCE END DATE: 02/28/2027

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