Monday, December 29, 2025 12/29/2025

3D Human Bone Marrow Microphysiological System Used to Investigate HSPC Mobilization via Inflammation-Derived ExtracellularVesicles

Award Number: F31AI189814
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 01/01/2025
PERIOD OF PERFORMANCE END DATE: 12/31/2027

Group Awards By:

View Award Description

3D Human Bone Marrow Microphysiological System Used to Investigate HSPC Mobilization via Inflammation-Derived ExtracellularVesicles - ABSTRACT The ultimate goal of this F31 Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship to Promote Diversity in Health-Related Research is to request support to address the mechanisms of HSPC and neutrophil mobilization from the bone marrow. The bone marrow microenvironment (BMM) is an intricate system composed of different cell types including osteoblasts, osteoclasts, mesenchymal stem cells, endothelial cells, fibroblasts, macrophages, and many others. This system plays a significant role in pathogenesis by regulating the production of blood cells to maintain homeostasis and responding to stress signals that contribute to cell production. In infections and cancer, there are changes in the BMM associated with HSPC and neutrophil proliferation. However, in both cases, the mechanisms driving the release of HSPC and neutrophil are only partially understood. Some studies suggest that the CXCR4/SDF-1 interaction between stromal cells, neutrophils and HSPCs retains these immune cells in the bone marrow and interaction with G-CSF mobilizes these cells. The primary goal of the project is to use our bone marrow microphysiological system (BM MPS), which I have proven produces neutrophils and HSPCs (preliminary data), to study the mobilization of HSPCs from the bone marrow microenvironment (BMM) in response to inflammation at distant sites. I will test the hypothesis that hematopoietic stem/progenitor cells (HSPCs) can be mobilized from the bone marrow in response to extracellular vesicles (EVs) derived from inflamed cells (derived from cancer and skin infections) . Understanding the mechanism that leads to HSPC mobilization is important as it is believed to play a significant role in tumor progression and hematopoiesis dysfunction in cancer and is thought to be a key mediator of innate immune response in skin and viral infections. The research milestones include: 1) Quantify number of HSPCs in BM MPS when dosed with G-CSF; 2) Isolate and characterize EVs derived from keratinocytes, dermal fibroblasts, and the MCF10A cell line series and confirm expression of G-CSF within these particles; and 3) Examine HSPC mobilization mechanism in the BM MPS by introducing inflammation derived EVs in the presence and absence of CSL324, a G-CSF antagonist. In addition to the research milestones, the proposal will seek to accomplish a series of milestones associated with my career development including: 1) prepare for my qualifying exam by attending seminars and lectures in biomedical research 2) continue to mentor undergraduate researchers; 3) present my work at the MPS World Summit, and the SACNAS conference; and 4) publish my work in a peer-reviewed archived journal(s); Accomplishing these milestones will equip me with the writing and project development skills necessary for a career as an independent investigator.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $42,622 )
2026	2026	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR STE 300	DAVIS	CA	95618	YOLO	USA	Allergy and Infectious Diseases Research	000	2	12/19/2025	NON-COMPETING CONTINUATION	$42,622
														Subtotal = $42,622

Issue Date FY: 2025 ( Subtotal = $42,622 )
2025	2025	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR STE 300	DAVIS	CA	95618	YOLO	USA	Allergy and Infectious Diseases Research	000	1	11/19/2024	NEW	$42,058
2025	2025	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR STE 300	DAVIS	CA	95618	YOLO	USA	Allergy and Infectious Diseases Research	002	1	7/25/2025	NEW	$564
2025	2025	UNIVERSITY OF CALIFORNIA, DAVIS	1850 RESEARCH PARK DR STE 300	DAVIS	CA	95618	YOLO	USA	Allergy and Infectious Diseases Research	001	1	11/19/2024	NEW	$0
														Subtotal = $42,622

Grand Total All Awards = $85,244

Top

All Categories

About

Search

Reports

Data Submission

Award Information

3D Human Bone Marrow Microphysiological System Used to Investigate HSPC Mobilization via Inflammation-Derived ExtracellularVesicles

Award Number: F31AI189814

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 01/01/2025

PERIOD OF PERFORMANCE END DATE: 12/31/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer