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Annotated lineage trees of murine development

Award Number: DP2GM149750
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2022
PERIOD OF PERFORMANCE END DATE: 08/31/2025

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Annotated lineage trees of murine development - Understanding the forces that direct cell fate is a central goal of biology. Many fundamental questions remain unanswered and vast tracts of vertebrate development are almost completely unexplored. What are the developmental origins and lineage relationships of cell types? What, where, and when are the key molecular events that underlie cell fate transitions? These questions remain unanswered as we lack the technology to map cell fate in both high-throughput and high-resolution. The overall objective of the proposed work is to develop and apply novel technologies that track and translate the cell fate forces into human-decipherable recordings. Our proposal combines innovative use of new CRISPR systems, molecular recording technologies, and improvements to our established lineage tracing technology to reconstruct high accuracy, annotated lineage trees. We will apply these cell fate technologies in mouse embryonic stem cells (mESCs), where we will track differentiation into various progeny cells, generating both transcriptional and lineage maps describing the probabilistic relationship between intestinal cell types at high-resolution. We will then expand these systems into recording mouse lines, where both molecular and lineage recordings can be captured from terminally differentiated cells. In aggregate, the resulting high-resolution lineage maps and tools will provide new insight into the plasticity of cell fate, and these recording mouse lines will be a valuable resource to the biomedical community. We believe these trailblazing technologies will ultimately spur the development of novel fate modulators with application to human disease.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2022	TRUSTEES OF DARTMOUTH COLLEGE	7 LEBANON ST	HANOVER	NH	03755	GRAFTON	USA	Trans-NIH Research Support	000	1	11/25/2025	NEW	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $981,250 )
2025	2025	TRUSTEES OF DARTMOUTH COLLEGE	7 LEBANON ST	HANOVER	NH	03755	GRAFTON	USA	Trans-NIH Research Support	000	2	8/27/2025	EXTENSION WITH OR WITHOUT FUNDS	$981,250
														Subtotal = $981,250

Issue Date FY: 2022 ( Subtotal = $1,476,000 )
2022	2022	TRUSTEES OF DARTMOUTH COLLEGE	7 LEBANON ST	HANOVER	NH	03755	GRAFTON	USA	Trans-NIH Research Support	000	1	8/24/2022	NEW	$1,476,000
														Subtotal = $1,476,000

Grand Total All Awards = $2,457,250

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Annotated lineage trees of murine development

Award Number: DP2GM149750

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2022

PERIOD OF PERFORMANCE END DATE: 08/31/2025

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