Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Decoding Cell-Fate Decisions Through Molecular Recording

Award Number: DP2EB041714
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2026
PERIOD OF PERFORMANCE END DATE: 07/31/2031

Group Awards By:

View Award Description

Decoding Cell-Fate Decisions Through Molecular Recording - Project Summary Deciphering how combinations of intrinsic and extrinsic cues determine cell fates is a fundamental challenge in developmental biology, with broad implications for regenerative medicine and cancer. While single-cell genomics technologies can profile cell states in detail, current approaches cannot reconstruct how prior events—such as signaling pathway activations, transient gene expression changes, or cell-cell interactions—shape developmental outcomes. Here we propose to create a new class of single-cell genomic recording technologies that integrate past cellular histories with present states captured by single-cell profiling. We will design modular synthetic circuits that permanently encode diverse biological events—lineage history, signaling activity, transient transcript expression, microenvironmental interactions, and CRISPR-based genetic perturbations—as retrievable DNA edits. These records will enable the retrospective reconstruction of developmental trajectories at single-cell resolution, revealing how combinations of past cellular events program final cell type. As a proof of concept, we will apply this system to the directed differentiation of human pluripotent stem cells into pancreatic islets, a clinically relevant model for treating Type 1 diabetes. This approach will reveal how transient cellular events affect cell-fate decisions and establish a broadly applicable platform for recording and reconstructing sequences of cellular events that determine the final cell fate.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $843,125 )
2026	2026	SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH	1275 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Trans-NIH Research Support	000	1	9/3/2026	NEW	$843,125
														Subtotal = $843,125

Grand Total All Awards = $843,125

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Decoding Cell-Fate Decisions Through Molecular Recording

Award Number: DP2EB041714

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2026

PERIOD OF PERFORMANCE END DATE: 07/31/2031

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer