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Engineering Bacteriophage T4 as a Targeted Gene Therapy Drug for in vivo HIV Cure

Award Number: DP1DA060580
ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/01/2024
PERIOD OF PERFORMANCE END DATE: 03/31/2029

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Engineering Bacteriophage T4 as a Targeted Gene Therapy Drug for in vivo HIV Cure - Project Summary/Abstract Successful execution of all the research aims in the parent award (1DP1DA060580-01) is critically dependent on continuous production of high-quality bacteriophage T4 capsid (head) nanoparticles, which serve as the core platform for engineering various therapeutic payloads for HIV cure. These nanoparticles, referred to as artificial viral vectors” or AVVs, enable delivery of in vitro-packaged reporter and therapeutic DNAs and surface-displayed genome editing molecules, cell-targeting molecules, and recombinases, into hematopoietic stem cells. Multiple steps in this process - virus production, capsid purification, and AVV construction - require high-speed centrifugation. The Sorvall RC-6 Plus centrifuge available in our laboratory is not able to meet these demands because it is both antiquated and currently inoperable. While repair may be possible, given its age and history of frequent breakdowns, it is likely to cause periods of workflow interruptions. Furthermore, the likelihood of the current centrifuge lasting through the entire award timeline is uncertain. This therefore represents a critical bottleneck that we need to address immediately. We request $94,755.54 in administrative supplement funds to acquire a modern LYNX 6000 high- speed centrifuge that is not only reliable but also comes with greater capacity and performance. For instance, the newer model has 6-liter capacity and 45% higher speeds that would allow us to generate larger quantities as well as more complete recovery of nanoparticles in each run. In addition, the LYNX 6000 carries safety features including rotor auto-detection, auto-locking, and a new structural design for ergonomic rotor installation. No carry-over funds are available to make this purchase. Thus, this administrative supplement, by addressing this technical challenge, will restore nanoparticle production, ensure continuity of critical workflows, and accelerate progress towards developing T4-based therapeutics for HIV cure.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $1,059,286 )
2026	2026	CATHOLIC UNIVERSITY OF AMERICA (THE)	620 MICHIGAN AVE NE	WASHINGTON	DC	20064	DISTRICT OF COLUMBIA	USA	Drug Use and Addiction Research Programs	000	3	3/27/2026	NON-COMPETING CONTINUATION	$964,530
2026	2026	CATHOLIC UNIVERSITY OF AMERICA (THE)	620 MICHIGAN AVE NE	WASHINGTON	DC	20064	DISTRICT OF COLUMBIA	USA	Drug Use and Addiction Research Programs	001	3	9/14/2026	SUPPLEMENT FOR EXPANSION	$94,756
														Subtotal = $1,059,286

Issue Date FY: 2025 ( Subtotal = $964,530 )
2025	2025	CATHOLIC UNIVERSITY OF AMERICA (THE)	620 MICHIGAN AVE NE	WASHINGTON	DC	20064	DISTRICT OF COLUMBIA	USA	Drug Use and Addiction Research Programs	001	2	4/14/2025	NON-COMPETING CONTINUATION	$96,453
2025	2025	CATHOLIC UNIVERSITY OF AMERICA (THE)	620 MICHIGAN AVE NE	WASHINGTON	DC	20064	DISTRICT OF COLUMBIA	USA	Drug Use and Addiction Research Programs	000	2	3/13/2025	NON-COMPETING CONTINUATION	$868,077
														Subtotal = $964,530

Issue Date FY: 2024 ( Subtotal = $964,530 )
2024	2024	CATHOLIC UNIVERSITY OF AMERICA (THE)	620 MICHIGAN AVE NE	WASHINGTON	DC	20064	DISTRICT OF COLUMBIA	USA	Drug Use and Addiction Research Programs	000	1	5/24/2024	NEW	$964,530
														Subtotal = $964,530

Grand Total All Awards = $2,988,346

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Engineering Bacteriophage T4 as a Targeted Gene Therapy Drug for in vivo HIV Cure

Award Number: DP1DA060580

ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/01/2024

PERIOD OF PERFORMANCE END DATE: 03/31/2029

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