Urban Indian Organization Diabetes and Obesity Prevention Capacity-Building Collaborative - The National Council of Urban Indian Health (NCUIH) proposes the national Urban Indian Organization (UIO) Diabetes and Obesity Prevention Capacity-Building Collaborative, a 24-month implementation cohort project designed to strengthen the capacity of participating UIOs to implement, evaluate, improve, disseminate, and sustain culturally grounded, evidence-informed obesity and diabetes prevention strategies for American Indian and Alaska Native peoples living in urban areas. While the project will support a cohort of participating UIOs to implement and evaluate these strategies, it is designed to generate practical tools, resources, and promising practices that can be shared and adapted across the Urban Indian Health System nationwide. With support from experienced subject matter expert UIOs, Indian Health Care Resource Center of Tulsa and Native Health of Phoenix, the project will provide structured technical assistance, workforce training, partnership development support, and practical implementation tools to expand nutrition education, physical activity, whole-food, diabetes prevention, family wellness, and related chronic disease prevention activities. Through this collaborative model, NCUIH will build community-level data and evaluation capacity, complete Health Outcomes and Population Needs Assessments, support quarterly reporting and quality improvement, and document promising practices that can be adapted across the national UIO network. Project findings will be disseminated through practical toolkits, case studies, webinars, NCUIH Annual Conference sessions, and public-facing resources to support Urban Indian Organizations nationwide. By the end of the project period, participating UIOs will have strengthened local prevention infrastructure, improved capacity to use data for program improvement, expanded partnerships and referral/resource pathways, and developed sustainability strategies to continue effective obesity and diabetes prevention efforts beyond the federal funding period. The resulting tools, resources, and lessons learned will strengthen obesity and diabetes prevention capacity throughout the Urban Indian Health System and contribute to long-term efforts to reduce the burden of chronic disease among American Indian and Alaska Native people living in urban areas nationwide.