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Large-Volume Dielectric Resonators for mm-Wave Pulsed EPR

Award Number: R01GM153951
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2024
PERIOD OF PERFORMANCE END DATE: 01/31/2028

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Large-Volume Dielectric Resonators for mm-Wave Pulsed EPR - Pulse Electron Paramagnetic Resonance (EPR) spectroscopy is a well-developed indispensable tool for solving complex problems in biochemistry and structural biology. Pulse EPR encompasses several established methods such as double electron-electron resonance (DEER), relaxation-induced dipolar modulation enhancement (RIDME), and double quantum coherence (DQC) spectroscopy that are uniquely suited for obtaining long-range (up to 10 nm or longer) distances and distance distributions between spin labeled sites in biomacromolecules. The distances between endogenous paramagnetic metal centers can also be determined. Another particularly impactful application of pulse EPR is in studies of metalloenzymes, where such methods as 3-pulse electron spin echo envelope modulation (ESEEM) spectroscopy, electron nuclear double resonance (ENDOR), hyperfine sublevel correlation spectroscopy (HYSCORE), and electron double resonance detected NMR (EDNMR) spectroscopy have evolved into highly informative techniques for providing mechanistic details on the function of many important metalloenzymes without the need of preparing protein crystals. However, for all these pulse methods insufficient concentration sensitivity remains the main roadblock towards their broader applicability in biochemical and biophysical research. The goal of this technology development project is to dramatically - by at least one to two orders of magnitude - increase concentration sensitivity of pulse W-band (94 GHz) EPR spectroscopy by developing large-volume, high-quality factor (high-Q), and high finesse microwave resonators based on low-loss dielectric materials. So far this new pulse EPR technology has been only demonstrated as a proof-of-principle and will require further substantial efforts including: (Aim 1) Design and optimization of high volume / high quality factor resonators for pulse EPR for the best concentration sensitivity and construction of the resonator prototypes; (Aim 2) Development of critical mm-wave instrumentation to implement high volume / high sensitivity resonators for routine and advanced W-band pulse EPR experiments; and (Aim 3) Validating the new technology using well studied and well characterized systems. The latter will be done by carrying out 14N ESEEM/HYSCORE experiments with Fe center in myoglobin and di- Mn complexes, evaluating 55Mn EDNMR sensitivity gains for a series of Mn ion complexes, including di-Mn clusters as well as S3 state of the oxygen evolving complex of PSI, and applying the DEER method to resolve distance and angular information for a series of rigid biradicals, transmembrane peptides and rhodopsin oligomers. If successful, this project will provide an innovative technology solution to the paramount long- standing issues of pulse EPR spectroscopy such as increasing both the excitation bandwidth and sensitivity at higher EPR frequencies. This will lead to a broader applicability of pulse EPR methods across the fields of biochemistry and structural biology.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $281,116 )
2026	2026	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	000	3	1/27/2026	NON-COMPETING CONTINUATION	$281,116
														Subtotal = $281,116

Issue Date FY: 2025 ( Subtotal = $536,009 )
2025	2025	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	000	2	1/17/2025	NON-COMPETING CONTINUATION	$482,409
2025	2025	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	001	2	8/12/2025	NON-COMPETING CONTINUATION	$53,600
														Subtotal = $536,009

Issue Date FY: 2024 ( Subtotal = $537,957 )
2024	2024	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	000	1	4/3/2024	NEW	$537,957
														Subtotal = $537,957

Grand Total All Awards = $1,355,082

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Large-Volume Dielectric Resonators for mm-Wave Pulsed EPR

Award Number: R01GM153951

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2024

PERIOD OF PERFORMANCE END DATE: 01/31/2028

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