Wednesday, February 18, 2026 2/18/2026

Assembly of the eukaryotic small ribosomal subunit

Award Number: R01GM145950
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/20/2022
PERIOD OF PERFORMANCE END DATE: 03/31/2025

Group Awards By:

View Award Description

Assembly of the eukaryotic small ribosomal subunit - Project Summary Ribosomes are molecular machines composed of ribosomal RNAs and up to 80 ribosomal proteins. These large assemblies catalyze protein synthesis in all cells. The long-term goal of this project is to understand how eukaryotic ribosomes are assembled with the help of more than 200 non-ribosomal factors as a series of molecular snapshots of assembly intermediates. Combining genetic, biochemical and mass spectrometry approaches with cryo-EM is an essential step to engineer, trap, isolate and determine atomic-resolution molecular snapshots of transient assembly intermediates of ribosomal subunits. Eukaryotic ribosome assembly can be subdivided into four stages, co-transcriptional assembly events and initial maturation of small and large ribosomal subunit precursors in the nucleolus, nuclear maturation of pre-40S and pre-60S particles, nuclear export, and cytoplasmic maturation. While late events in eukaryotic ribosome assembly are relatively well characterized, the early assembly of ribosomal subunits in the nucleolus is still poorly understood. Especially the mechanisms responsible for early human small ribosomal subunit assembly have remained elusive due to a biomolecular condensate that retains these early particles in human nucleoli. This proposal describes new approaches to define the molecular mechanisms that govern nucleolar maturation of the eukaryotic small ribosomal subunit. My laboratory has developed new human genome editing and biochemical approaches that now enable us to efficiently tag and isolate early nucleolar assembly intermediates of the human small ribosomal subunit. The synergistic use of these approaches has allowed us to overcome previously intractable biochemical hurdles, thereby enabling the detailed study of essential early assembly intermediates of the small ribosomal subunit. Insights from these studies will shed light onto both the mechanisms that are employed during eukaryotic ribosome assembly to coordinate key processing events as well as how defects in eukaryotic ribosome assembly can result in human blood disorders, which are collectively termed ribosomopathies.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	001	3	7/18/2025	NON-COMPETING CONTINUATION	$0
2025	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	3	4/9/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $339,000 )
2024	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	001	3	7/12/2024	NON-COMPETING CONTINUATION	$33,900
2024	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	3	3/22/2024	NON-COMPETING CONTINUATION	$305,100
														Subtotal = $339,000

Issue Date FY: 2023 ( Subtotal = $339,000 )
2023	2023	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	2	3/21/2023	NON-COMPETING CONTINUATION	$339,000
														Subtotal = $339,000

Issue Date FY: 2022 ( Subtotal = $339,000 )
2022	2022	ROCKEFELLER UNIVERSITY, THE	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	1	4/20/2022	NEW	$339,000
														Subtotal = $339,000

Grand Total All Awards = $1,017,000

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Assembly of the eukaryotic small ribosomal subunit

Award Number: R01GM145950

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/20/2022

PERIOD OF PERFORMANCE END DATE: 03/31/2025

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer