Tuesday, February 17, 2026 2/17/2026

The role of macrophages in chronic suppurative otitis media associated sensory hearing loss

Award Number: R01DC019965
ORGANIZATION: NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/01/2025
PERIOD OF PERFORMANCE END DATE: 12/31/2026

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The role of macrophages in chronic suppurative otitis media associated sensory hearing loss - Project Summary / Abstract We request NIH support to investigate how sensory hearing loss (SHL) is caused by chronic suppurative otitis media (CSOM) or severe chronic middle ear infections. CSOM, a neglected tropical disease that afflicts 330 million people worldwide, is the most common cause of permanent hearing loss among children in the developing world. It is characterized by a chronically discharging infected middle ear, and there is currently no effective medical therapy or cure. The bacterium, Pseudomonas aeruginosa (PA), is the leading culprit. PA colonizes the middle ear via a hole in the tympanic membrane and establishes itself into a biofilm community, complicating attempts to treat and fully eradicate infection. Over the course of the disease, the infection waxes and wanes as the population of bacteria within the biofilm responds, in part, to immune attack or topical antibiotics. This waxing and waning of bacterial infection leads to permanent sensory hearing loss via an unknown mechanism. Our lab has recently created and validated a novel PA CSOM animal model that mimics the human condition. Specifically, we create the infection by inoculating PA in the right state (phenotype) and dose, which results in an infection that persists beyond six months, waxes and wanes upon topical fluoroquinolone therapy, and leads to hair cell death, over time, like in the human disease. Previous investigations by others relied on acute infection models based on non-PA bacteria. In contrast, our unique model of PA CSOM now allows us to observe development of the infection in the inner ear and identify agents and/or processes that may be causing the resulting sensory hearing loss. Our studies would help determine whether permanent hearing loss is preventable in CSOM and, if so, guide strategies for therapeutic intervention. Our Aims encompass: (1) determining the timing and nature of structural changes occurring within the cochlea and assessing the macrophage distribution as these changes occur, (2) investigating potential direct hair cell (HC) toxins and macrophage inducers through CSOM perilymph sampling, and (3) evaluating the contributions of resident and migrating macrophages towards hair cell loss in CSOM by combining our CSOM mouse model with the CD68-GFP transgenic reporter mouse and, separately, with a triple knockout mouse strain with macrophages unable to produce reactive oxygen species (ROS) while also evaluating whether NLRP3 inflammasome function is necessary for HC loss in the cochlea. Altogether these aims are a completely new approach to sensory hearing loss caused by CSOM. If successful, these studies will support future investigations into the mechanisms in the pathophysiology of CSOM and lead to novel treatments for PA and potential strategies to prevent sensory hearing loss in CSOM.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $726,188 )
2026	2026	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	001	5	2/11/2026	NON-COMPETING CONTINUATION	$72,618
2026	2026	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	000	5	1/22/2026	NON-COMPETING CONTINUATION	$653,570
														Subtotal = $726,188

Issue Date FY: 2025 ( Subtotal = $653,571 )
2025	2025	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	002	4	1/8/2025	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
2025	2025	UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION	4200 FIFTH AVENUE	PITTSBURGH	PA	15260	ALLEGHENY	USA	Research Related to Deafness and Communication Disorders	001	4	11/25/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$653,571
2025	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	000	3	11/22/2024	NON-COMPETING CONTINUATION	$0
2025	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	003	3	3/3/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $653,571

Issue Date FY: 2024 ( Subtotal = $743,721 )
2024	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	001	3	5/3/2024	NON-COMPETING CONTINUATION	$74,372
2024	2024	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	000	3	1/5/2024	NON-COMPETING CONTINUATION	$669,349
														Subtotal = $743,721

Issue Date FY: 2023 ( Subtotal = $773,654 )
2023	2023	THE LELAND STANFORD JUNIOR UNIVERSITY	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	002	2	4/26/2023	SUPPLEMENT FOR EXPANSION	$91,202
2023	2023	LELAND STANFORD JUNIOR UNIVERSITY, THE	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	001	2	1/14/2023	NON-COMPETING CONTINUATION	$68,244
2023	2023	LELAND STANFORD JUNIOR UNIVERSITY, THE	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	000	2	12/6/2022	NON-COMPETING CONTINUATION	$614,208
														Subtotal = $773,654

Issue Date FY: 2022 ( Subtotal = $682,447 )
2022	2022	LELAND STANFORD JUNIOR UNIVERSITY, THE	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	000	1	12/24/2021	NEW	$580,081
2022	2022	LELAND STANFORD JUNIOR UNIVERSITY, THE	450 JANE STANFORD WAY	STANFORD	CA	94305	SANTA CLARA	USA	Research Related to Deafness and Communication Disorders	001	1	1/25/2022	NEW	$102,366
														Subtotal = $682,447

Grand Total All Awards = $3,579,581

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The role of macrophages in chronic suppurative otitis media associated sensory hearing loss

Award Number: R01DC019965

ORGANIZATION: NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/01/2025

PERIOD OF PERFORMANCE END DATE: 12/31/2026

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