PROJECT SUMMARY/ABSTRACT
We are proposing the acquisition of a Chromium Connect System. The proposed instrument will
be placed in a shared setting at the Stanford Center for Genomics and Personalized Medicine
(SCGPM) Sequencing Service Center and operated by a highly trained group of staff experienced
in genomic technologies. The SCGPM Sequencing Service Center is a core facility that was
founded in 2009 and is open to the entire Stanford community. Since its inception the SCGPM
Sequencing Service Center has produced sequencing data for over 700 Stanford researchers
and more than 150 laboratory groups. Single cell sequencing has become a transformative tool
in the biomedical research space and the rapid decline in the cost of sequencing has generated
a need for consistent and reliable high-throughput library preparation. The presence of a
Chromium Connect will greatly increase the library preparation capabilities of the center as well
as shorten turnaround times for preparing libraries. The Major and Minor Users highlighted in this
proposal range from junior faculty who are just starting their own laboratories to established full
professors with over 30 years of research experience. Nearly all of the users have NIH funding,
and their genomic studies depend on affordable and customizable ultra high-throughput, next
generation sequencing technologies. We predict that use of the Chromium Connect System will
approach maximum capacity due to the high demand and need for cost-effective library
preparation. Data generated by sequencing libraries prepared with the proposed instrument will
positively impact a broad cross section of human disease research, notably: cardiovascular
disease, pulmonary hypertension, type 2 diabetes mellitus, inflammatory bowel diseases,
developmental defects, cancer, sleep disorders, obesity, and aging. The research performed in
these studies will help pave the way for better interpretations of the human genome as well as
the translation of new assays and technologies to the clinic. Stanford researchers who perform
single cell sequencing assays made possible by library preparation performed on this instrument
consistently publish high profile studies, indicating the major impact that this new technology will
have on biomedical research.