ABSTRACT:
We are requesting funds for a Seahorse XFe24 automated cellular respiration and
glycolysis analyzer. This instrument is the only instrument on the market that can assess
cellular respiration and glycolysis in physiologically relevant cellular models, through
non-invasive measurements that provide real time kinetic results. The Seahorse XFe24
will be placed within the shared resource core in the Basic Science Department at
NYUCD. The institution will support the instrument and the instrument internal review
board will administer instrument oversight. We also request the BioTek Cytation1 Multi
Mode Reader for assay normalization. This reader is linked to the Seahorse XFe24 and
is operated via unified controller, controlling both BioTek and Seahorse instrument.
The major user group spans a few departments at NYUCD and represents both basic
and translational research areas such as aging, cancer, diabetes, immunology, and
mitochondrial disorders. The NIH funded major users combined will use ~75% of the
instrument capacity. Remaining instrument capacity will be made available to any
internal or external researcher through an on-line scheduler.
The availability of this instrument will meet the specific needs of NYUCD researchers to:
1) monitor attached cells (a crucial consideration for our major users using osteoblasts,
osteocytes, ameloblasts, odontoblasts, neurons, and cancer cells), 2) obtain reliable
measurements from small numbers of cells (crucial for our users that derive primary
cells from mouse genetic models) and, 3) enable screening responses to a wide range
of physiologic signals, environmental insults, and genetic alterations in a high throughput
format.
Currently, our studies using Seahorse XFe24 analyzer for primary cells are done at the
NYU School of Medicine, which is ~10 city blocks away from NYUCD. This situation
requires transportation of cells and reagents across the city streets and significantly
compromises our experiments. Further, the XFe24 analyzer at the NYU School of
Medicine serves over 60 investigators and its usage is available from 9AM-5PM during
business days, which significantly limit our use time and flexibility in experimental
design.
Availability of Seahorse XFe24 analyzer will facilitate the research at NYUCD and
support the collection of outstanding NIH funded projects specified in this application.