Saturday, November 23, 2024
11/23/2024
NOT-GM-24-021 – ABSTRACT – HART Highly multiplexed genetic perturbations offer huge potential beyond fitness screens. High throughput microscopy offers a tractable method for combining complex genetic perturbation with multiparameter functional assays. Here we propose the purchase of a Nikon Eclipse Ji microscopy system to facilitate complex data collection to decipher the complexity and redundancy of mammalian signaling pathways. Using our next- generation CRISPR/Cas12a polygenic knockout system, we combine custom CRISPR arrays targeting signaling effectors and cell surface “barcodes”, then use immunofluorescence readouts of cell state across dozens of targets – two to four per well in a 96 well plate -- to understand the dynamics of cell signaling.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).
