Thursday, April 25, 2024
4/25/2024
PROJECT SUMMARY (Description) Perivascular fibroblasts (PVFs) and vascular endothelium are important responders to hypertension and cerebral ischemia. Long-term aberrant interactions of PVFs and endothelium may also promote vascular cognitive disorders (VCD), but the mechanisms remain poorly understood. This project will use PVF-specific single-cell RNA-Seq and vascular endothelium-specific or TRAP-Seq to test the impacts of hypertension and cerebral ischemia on gene expression. Outcomes of this project may suggest novel therapeutic targets. Aim 1 will test the transcriptome changes of endothelial cells in a mouse model of chronic hypertension (ReninTg) with and without partial cerebral ischemia. Aim 2 will use bitransgenic ReninTg and Pdgfra-H2BGFP mice, with and without partial cerebral ischemia, to isolate PVFs for single-cell RNA-Seq comparison. In summary, this project will use open-ended genomic methods to correlate, for the first time, the transcriptome adaptations by PVFs and vascular endothelium to hypertension and combined hypertension/ischemia.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).
