Abstract
During the past 30 years, notable advances have been made in understanding the etiologic agent, Borrelia
burgdorferi (Bb), and the illness that it causes. While ~30,000 cases are reported to the CDC each year, the
overall number of Bb-infected patients in the US is estimated to approach 400,000, suggesting that Lyme
disease is becoming an epidemic. Signs and symptoms of infection range in severity and most patients recover
fully after antimicrobial treatment; however, chronic serious illness and even deaths can still occur. Lyme
disease is usually diagnosed by clinical observation of erythema migrans (bulls-eye rash), however, some
patients do not develop erythema migrans. Why certain patients have no accompanying symptoms at time of
presentation whereas others have numerous symptoms has not been elucidated. Many studies have been
published detailing progress in expanding the knowledge base on the microbiology of Bb and on the ecology
and epidemiology, pathogenesis and clinical aspects but laboratory-based diagnosis of Lyme disease is
severely lagging behind.
It is been estimated that more than 2.7 million serum samples are tested each year for the presence of Borrelia
burgdorferi-specific antibodies in the United States alone. To meet the demand for laboratory-based diagnosis,
various new tests for direct detection of the etiologic agent, or for detection of post-infection specific antibodies
by using whole-cell lysates, recombinant antigens, or peptide antigens in enzyme immunoassays (EIA), have
been introduced into the clinical laboratory. However, the currently available Lyme disease diagnostics do not
meet the specifications for an ideal test, which would be rapid, sensitive, specific, and point-of-care. Currently,
the two step FDA approved diagnostic test will only detect the post-infection immune response to Lyme
disease pathogen, Bb, and with limited sensitivity and specificity. An ideal Lyme disease diagnostic is a test
that is specific for Bb, simple, non-invasive and relies only on readily available samples such as blood or urine.
Our proposal will provide specific targets detectable in blood at the earliest time point upon Bb infection. To
develop these tests, we have constructed an extensive proteogenomic resource of Bb isolates and provide a
publically accessible “Borrelia PeptideAtlas. Using our novel study design incorporating broad quantitative
surface exposed pathogen protein detection and comprehensive targeted peptide quantitation in complex host
proteome backgrounds, we will deploy novel highly specific and high sensitivity diagnostic assays targeting Bb
protein remnants for both early and treatment phase Lyme disease detection. The outcome of this proposal will
have great impact by reducing disproportionate determinations of Lyme disease through the establishment of
novel biomarkers for diagnostic test development in early stage Lyme disease.