Tuesday, December 2, 2025 12/2/2025

Lineage tracing origins of human innate lymphoid cells from hematopoietic precursors

Award Number: R03AI187610
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 11/19/2024
PERIOD OF PERFORMANCE END DATE: 10/31/2026

Group Awards By:

View Award Description

Lineage tracing origins of human innate lymphoid cells from hematopoietic precursors - Project summary Human natural killer (NK) cells are critical for control of viral infection and their importance for human health is underscored by the disease that results from dysregulated NK cell development or function. In the adult, NK cells are continuously generated from stem cells, making their generation an important process for human immunity. Despite the importance of this continuous generation, there is much that is still unknown about how stem cells with the potential to become any blood cell are directed to become an NK cell. This process is further complexified by the observation that myeloid progenitors can give rise to NK cells under certain conditions. Here we propose to address these questions using genetic ‘barcoding’ of human pluripotent stem cells combined with their differentiation to NK cell lineages. This experimental approach will be used to delineate the emergence of innate lymphoid cells from stem cells with single cell resolution. Aim 1 will optimize a novel genetic cell tracing system and use it to generate transcriptomic trajectories of NK cell differentiation from lymphoid or myeloid committed progenitors. Aim 2 will pair this reporter system with live cell imaging of cell dynamics as NK cells undergo differentiation to understand the dynamic interactions that give rise to human NK cells. Together, these aims will define the emergence of NK cells from multipotent progenitors and provide a mechanistic, single cell resolution understanding of how lymphoid and myeloid progenitors give rise to functionally mature innate lymphocytes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $82,250 )
2026	2026	THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK	630 W 168TH ST FL 4	NEW YORK	NY	10032	NEW YORK	USA	Allergy and Infectious Diseases Research	000	2	11/26/2025	NON-COMPETING CONTINUATION	$82,250
														Subtotal = $82,250

Issue Date FY: 2025 ( Subtotal = $79,783 )
2025	2025	THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK	630 W 168TH ST FL 4	NEW YORK	NY	10032	NEW YORK	USA	Allergy and Infectious Diseases Research	000	1	11/19/2024	NEW	$79,783
														Subtotal = $79,783

Grand Total All Awards = $162,033

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Lineage tracing origins of human innate lymphoid cells from hematopoietic precursors

Award Number: R03AI187610

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 11/19/2024

PERIOD OF PERFORMANCE END DATE: 10/31/2026

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer