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Lysine acetylation of ribosomal proteins during poliovirus infection limits viral translation

Award Number: R03AI144839
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

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Ribosomal proteins contain numerous posttranslational modifications, but the functions these ribosome modifications play during mRNA translation are currently unknown. Our long-term goal is to study the function of these posttranslational modifications, specifically how they regulate protein biosynthesis. We have found that ribosomal protein acetylations are altered during infection with poliovirus. Mutagenesis of some acetylated lysine residues in eS25, a protein required for poliovirus translation, benefits the virus, suggesting that some acetylations might have antiviral function. The objective of this grant is to identify the acetyltransferase, which modifies ribosomal proteins during poliovirus infection. The central hypothesis is that the same enzyme might target multiple ribosomal proteins, thus, we expect that overexpression or depletion of the acetyltransferase may have a cumulative and pronounced effect on poliovirus translation. Our specific aim will test our central hypothesis by depleting or overexpressing different acetyltransferases and determining the effect on poliovirus translation and ribosome modifications by immunoblotting and mass spectrometry analysis. Our proposal describes a significant paradigm shift from ribosomes as non-discriminatory translation machines, defenseless to viral takeover, to active regulators of protein biosynthesis with antiviral function to protect cells.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2021	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	1400 WASHINGTON AVE	ALBANY	NY	12222	ALBANY	USA	Allergy and Infectious Diseases Research	000	2	2/24/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $77,250 )
2021	2021	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	1400 WASHINGTON AVE	ALBANY	NY	12222	ALBANY	USA	Allergy and Infectious Diseases Research	000	2	1/26/2021	NON-COMPETING CONTINUATION	$77,250
														Subtotal = $77,250

Issue Date FY: 2020 ( Subtotal = $77,250 )
2020	2020	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	1400 WASHINGTON AVE	ALBANY	NY	12222	ALBANY	USA	Allergy and Infectious Diseases Research	000	1	1/31/2020	NEW	$77,250
														Subtotal = $77,250

Grand Total All Awards = $154,500

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Lysine acetylation of ribosomal proteins during poliovirus infection limits viral translation

Award Number: R03AI144839

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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