Wednesday, April 24, 2024 4/24/2024

Deciphering the regulatory code that specifies different cell fates in development using single cell genomics

Award Number: R01HD100035
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

View Award Abstract

Summary The vertebrate embryo contains hundreds of functionally diverse cell types, however we lack a complete understanding of the regulatory code that specifies each cell type and how disruptions in this code affect relationships between lineages. Here, we propose two aims to dissect the accessible regions of the chromatin, the regulatory motifs and the putative transcription factors that mediate the specification of multiple cell types during embryogenesis. First, (Aim 1), we will utilize single cell ATAC-seq and scRNA-seq to uncover the dynamics of chromatin accessibility and the transcriptional profiles that define discrete cell types during early development. We will define the regulatory motifs in accessible regions of the chromatin that are preferentially engaged in different cell types. Second (Aim 2), we will use manifold learning approaches to define co- regulated gene modules and apply a regulatory network inference framework for scRNA-seq and scATAC-seq data, and test the regulatory interactions by mutating key pioneer factors and investigate how they cooperate with other TFs to regulate chromatin accessibility and cell differentiation during development. This project will generate extensive, high-quality datasets as well as novel computational methods to enable quantitative and predictive models of embryonic development with the goal to decipher the gene regulatory network specifying cellular identity during development. Given that early development is conserved across vertebrate species, our findings have the potential to inform human development, and lay the foundation to investigate the etiologies of human developmental disorders and to engineer novel cell types and lineages in vitro for regenerative medicine.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $504,341 )
2024	2024	YALE UNIV	150 MUNSON ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	000	5	2/29/2024	NON-COMPETING CONTINUATION	$504,341
														Subtotal = $504,341

Issue Date FY: 2023 ( Subtotal = $560,379 )
2023	2023	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	000	4	2/23/2023	NON-COMPETING CONTINUATION	$560,379
														Subtotal = $560,379

Issue Date FY: 2022 ( Subtotal = $560,378 )
2022	2022	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	001	3	4/27/2022	NON-COMPETING CONTINUATION	$56,041
2022	2022	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	000	3	2/24/2022	NON-COMPETING CONTINUATION	$504,337
														Subtotal = $560,378

Issue Date FY: 2021 ( Subtotal = $560,378 )
2021	2021	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	001	2	4/2/2021	NON-COMPETING CONTINUATION	$0
2021	2021	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	000	2	3/2/2021	NON-COMPETING CONTINUATION	$560,378
														Subtotal = $560,378

Issue Date FY: 2020 ( Subtotal = $571,816 )
2020	2020	YALE UNIVERSITY	105 WALL ST	NEW HAVEN	CT	06511	NEW HAVEN	USA	Child Health and Human Development Extramural Research	000	1	5/7/2020	NEW	$571,816
														Subtotal = $571,816

Grand Total All Awards = $2,757,292

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Deciphering the regulatory code that specifies different cell fates in development using single cell genomics

Award Number: R01HD100035

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer