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Non-canonical EphB2-mediated signaling in AKI and its progression to CKD

Award Number: R01DK144987
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/13/2026
PERIOD OF PERFORMANCE END DATE: 01/31/2031

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Non-canonical EphB2-mediated signaling in AKI and its progression to CKD - Transforming growth factor (TGF)-β promotes inflammatory and fibrotic responses via activation of the serine/threonine kinase receptor TβRII and consequent activation of SMAD3. In the kidney, TGF-β is activated in acute kidney injury (AKI) and is associated with proximal tubule epithelial cell (PTEC) responses that lead to the development of and progression to chronic kidney disease (CKD). To this end, PTECs produce inflammatory cytokines, extracellular matrix, and stimulate differentiation of fibroblasts into myofibroblasts, the major producers of extracellular matrix and drivers of fibrosis. Although a role for TGF-β in AKI and CKD has been established, anti-TGF-β therapy has not yet translated into successful treatment in CKD patients. A possible explanation is that TβRII can be activated in a ligand-independent manner which potentiates low dose TGF-β signaling. We showed that the cytoplasmic tail of TβRII contains several tyrosines which can be phosphorylated in a TGF-β independent manner, leading to SMAD3 activation and fibrotic responses in kidney cells. We found that the tyrosine kinase EPH receptor B2 (EphB2) forms a complex with and phosphorylates TβRII. EphB2 is expressed by PTECs and it contributes to organ injury by promoting inflammation and fibrosis. However, the contribution of EphB2 to AKI-to-CKD progression and the cells responsible for its deleterious effects have not been investigated. Using a model of AKI that progresses to CKD, we show that pYEphB2 and pYTβRII are evident in the acute and chronic phases of kidney injury. Ephrin B1-mediated activation of EphB2 leads to pYTβRII and enhances TGF- β-induced SMAD3 activation and epithelial-to-mesenchymal transition. Mice treated with a novel EphB2 inhibitor showed reduced tubular injury, inflammation, collagen production as well as pYEphB2 and pYTβRII following ischemia/reperfusion-induced AKI. Interestingly, we found that macrophage inhibitor factor (MIF), a cytokine that contributes to inflammation following kidney injury, is produced by PTECs when stimulated with Ephrin B1, but not TGF-β. Thus, there is a canonical EphrinB1/EphB2 as well as a non-canonical EphrinB1/EphB2/TGF-β axis in PTECs that can contribute to inflammation and fibrosis in AKI and its progression to CKD. We hypothesize that EphB2 expressed by PTECs contributes to inflammation and fibrosis following AKI and its progression to CKD by inducing the secretion of MIF and by potentiating TGF-β signaling via phosphorylation of TβRII. In this grant, we will: Determine the contribution of PTECs in EphB2-induced kidney inflammation and fibrosis (Aim 1). Determine the mechanisms whereby EphB2 contributes to inflammation and fibrosis (Aim 2). Determine whether the EphB2 small molecular weight inhibitor is beneficial in AKI and its progression to CKD (Aim 3). This study will identify a novel mechanism whereby EphB2 and TβRII interact; how EphB2/TβRII-mediated fibrotic signaling can be modulated in kidney disease; and devise new therapeutic interventions aimed to prevent EphB2 activation and function in kidney disease.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $866,265 )
2026	2026	VANDERBILT UNIVERSITY MEDICAL CENTER	1161 21ST AVE S STE D3300 MCN	NASHVILLE	TN	37232	DAVIDSON	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	5/13/2026	NEW	$866,265
														Subtotal = $866,265

Grand Total All Awards = $866,265

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Non-canonical EphB2-mediated signaling in AKI and its progression to CKD

Award Number: R01DK144987

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/13/2026

PERIOD OF PERFORMANCE END DATE: 01/31/2031

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