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Structural basis of the HIV-1 PBS-segment mediated RHA recruitment during assembly to bolster virus infectivity

Award Number: R01AI150460
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

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Project Summary RNA Helicase A (RHA) is one of the cellular factors recruited into HIV-1 particles during virus assembly. The reduced infectivity of RHA-deficient virions demonstrates that the RHA molecules co-assembled with gRNA exert a continuous impact in early infection. However, it remains unclear how host RHA is specifically recruited during virus assembly, and how it supports the infectivity of progeny virions. Our laboratory has recently reported that the recruitment of RHA is mediated by the direct interactions between RHA and the PBS-segment of the viral genomic RNA during assembly. In addition, our preliminary studies indicate that the specific RHA:PBS-segment interaction may coordinate proper tRNA placement on the PBS-segment to initiate reverse transcription. We propose to employ an innovative integrated NMR/SAXS approach, in combination with cell-based functional assays, to determine the structural basis of the PBS-segment mediated recruitment of RHA during virus assembly, and to investigate the role of RHA during the early stage of viral replication.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	UNIVERSITY OF MISSOURI SYSTEM	121 UNIVERSITY HALL	COLUMBIA	MO	65211	BOONE	USA	Biomedical Research and Research Training	000	5	3/12/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $305,494 )
2021	2021	UNIVERSITY OF MISSOURI SYSTEM	316 UNIVERSITY HALL	COLUMBIA	MO	65211	BOONE	USA	Biomedical Research and Research Training	000	5	6/14/2021	NON-COMPETING CONTINUATION	$305,494
														Subtotal = $305,494

Issue Date FY: 2020 ( Subtotal = $305,494 )
2020	2020	UNIVERSITY OF MISSOURI SYSTEM	316 UNIVERSITY HALL	COLUMBIA	MO	65211	BOONE	USA	Biomedical Research and Research Training	000	4	6/30/2020	NON-COMPETING CONTINUATION	$305,494
														Subtotal = $305,494

Issue Date FY: 2019 ( Subtotal = $305,494 )
2019	2019	UNIVERSITY OF MISSOURI SYSTEM	316 UNIVERSITY HALL	COLUMBIA	MO	65211	BOONE	USA	Biomedical Research and Research Training	000	3	6/18/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$305,494
														Subtotal = $305,494

Grand Total All Awards = $916,482

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Structural basis of the HIV-1 PBS-segment mediated RHA recruitment during assembly to bolster virus infectivity

Award Number: R01AI150460

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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