Thursday, April 25, 2024 4/25/2024

Defining the role of post-translational regulation by extracellular proteases in the pathogenesis of Staphylococcus aureus osteomyelitis

Award Number: R01AI119380
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

View Award Abstract

SUMMARY ABSTRACT We have demonstrated that mutation of the staphylococcal accessory regulatory (sarA) in Staphylococcus aureus results in an increase in the production of extracellular proteases to a degree that limits biofilm formation, limits cytotoxicity for mammalian cells including osteoblasts and osteoclasts, and limits the accumulation of both surface-associated and extracellular virulence factors. We have also demonstrated that this can be correlated with decreased virulence in animal models of sepsis and osteomyelitis. Moreover, we have confirmed that all of these phenotypes are evident in diverse clinical isolates of S. aureus and that they can be reversed by eliminating the ability of sarA mutants to produce extracellular proteases. In this proposal, we will expand on these observations to identify the specific extracellular proteases that are most relevant in the context of our underlying scientific hypothesis in diverse clinical isolates of S. aureus (Aim 1) and use the information gained to interrogate the impact of these proteases on the virulence factor repertoire on these clinical isolates, define the impact sarA and these proteases on bone remodeling and the host response in bone infection, and ultimately identify and evaluate the contribution of specific S. aureus virulence factors alone and in combination with each other on these phenotypes (Aim 2).


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $452,088 )
2023	2023	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	001	8	7/27/2023	NON-COMPETING CONTINUATION	$452,017
2023	2019	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	5	11/23/2022	NON-COMPETING CONTINUATION	$71
														Subtotal = $452,088

Issue Date FY: 2022 ( Subtotal = $452,043 )
2022	2022	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	004	7	8/2/2022	NON-COMPETING CONTINUATION	$452,043
2022	2021	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	003	6	6/15/2022	COMPETING CONTINUATION	$78
2022	2021	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	001	6	6/14/2022	COMPETING CONTINUATION	$216,754
2022	2019	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	002	5	6/15/2022	NON-COMPETING CONTINUATION	-$78
2022	2019	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	5	6/14/2022	NON-COMPETING CONTINUATION	-$216,754
														Subtotal = $452,043

Issue Date FY: 2021 ( Subtotal = $451,808 )
2021	2021	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	002	6	9/23/2021	COMPETING CONTINUATION	$451,808
2021	2019	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	001	5	9/22/2021	NON-COMPETING CONTINUATION	$0
2021	2019	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	5	3/29/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $451,808

Issue Date FY: 2019 ( Subtotal = $369,022 )
2019	2019	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	5	5/3/2019	NON-COMPETING CONTINUATION	$369,022
														Subtotal = $369,022

Issue Date FY: 2018 ( Subtotal = $369,022 )
2018	2018	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	4	5/10/2018	NON-COMPETING CONTINUATION	$369,022
														Subtotal = $369,022

Issue Date FY: 2017 ( Subtotal = $369,022 )
2017	2017	UNIVERSITY OF ARKANSAS SYSTEM	4301 W MARKHAM ST SLOT 812 BIO-MED BLDG 1 STE 102	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	3	5/16/2017	NON-COMPETING CONTINUATION	$369,022
														Subtotal = $369,022

Issue Date FY: 2016 ( Subtotal = $369,022 )
2016	2016	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST, M S 545	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	2	5/10/2016	NON-COMPETING CONTINUATION	$369,022
														Subtotal = $369,022

Issue Date FY: 2015 ( Subtotal = $369,022 )
2015	2015	UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES	4301 W MARKHAM ST, M S 545	LITTLE ROCK	AR	72205	PULASKI	USA	Allergy and Infectious Diseases Research	000	1	6/15/2015	NEW	$369,022
														Subtotal = $369,022

Grand Total All Awards = $3,201,049

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Defining the role of post-translational regulation by extracellular proteases in the pathogenesis of Staphylococcus aureus osteomyelitis

Award Number: R01AI119380

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer