HHS Recovery Act Recipient Reporting Readiness Tool

Step 4. Review and Copy the Grant Awards Data

TAGGS provides some – but not all – of the data needed for the Recipient Report. Recipients are responsible for directly collecting and reporting all required data to FederalReporting.gov. Data that HHS does not currently collect are highlighted in yellow. Do not copy this highlighted information. Please enter the appropriate data for your organization in these required fields. For assistance with entering these data please contact FederalReporting.gov.

You may capture the data HHS does provide by copying data from this screen and pasting it into the reporting format of your choice, such as the Excel spreadsheet template, the XML template, or by logging into the online form. For assistance with copying and pasting these data please email our help desk at Readiness Help

Prime Recipient Report

Award Detail for: COLON CELL DIFFERENTIATION: NAB AND MUC2 GENE EXPRESSION
Recipient Name:MONTEFIORE MEDICAL CENTER
DUNS Number: 041581026
111 EAST 210TH STREET
BRONX, NY 10467-2401

Reporting Information

Award Type*: Grant

Award Number*: 3R01DK058245-05S1

Final Report*: Recipient responsible for this data

Award Recipient Information

Recipient DUNS Number*: 041581026

Recipient Account Number: Recipient responsible for this data

Recipient Congressional District*: 17

Award Information

Funding Agency Code*: 7529

Awarding Agency Code*:7529

Award Date*: 09-16-2009

Amount of Award*: $ 50,000

Program Source (TAS)*: 750883

CFDA Number*: 93.701

Sub Account Number for Program Source (TAS)*: Recipient responsible for this data

Total Number of Sub Awards to Individuals*: Recipient responsible for this data

Total Amount of Sub Awards to Individuals*: Recipient responsible for this data

Total Number of Payments to Vendors less than $25,000/award*: Recipient responsible for this data

Total Amount of Payments to Vendors less than $25,000/award*: Recipient responsible for this data

Total Number of Sub Awards less than $25,000/award*: Recipient responsible for this data

Total Amount of Sub Awards less than $25,000/award*: Recipient responsible for this data

Award Description* DESCRIPTION (provided by applicant): The short chain fatty acid butyrate (NaB), present in high concentrations in the colonic lumen principally from the fermentation of dietary fiber, plays a physiological role in colonic homeostasis. This involves maintaining an equilibrium not only between cell proliferation in the lower section of the crypt and cell loss at the top of the crypt, but also a balance among the multiple cell lineages in the mucosa. However, butyrate effects are complex, in that butyrate induces differentiation in vitro, but promotes growth in vivo. This complexity is further emphasized by our findings demonstrating that NaB specifically represses that expression of the MUC2 gene, which encodes the major colonic mucin, and does so by inhibiting HDAC activity (Oncogene, in press). It is our hypothesis that the intestine has developed specific mechanisms to respond to natural compounds, such as NaB, present in the lumen to modulate cell differentiation as a function of cell position along the crypt axis. Alterations in this balance may increase the probability of tumor formation. Utilizing cell systems that recapitulate the goblet and ion transporter phenotypes, and molecular reagents developed in the lab, we will define the molecular determinants in cell lineage specific differentiation that can be regulated by NaB by determining: 1. Whether there are NaB responsive elements in the promoter of the human and mouse MUC2 genes. 2. Whether HDAC activity can counteract NaB mediated repression of MUC2 3. Whether there is modulation of SP1/SP3 activity, which we demonstrated is important for MUC2 regulation. 4. Whether post-translational modifications of SP1/SP3 may bring about loss of transcriptional activity. This will be done in vitro using standard immunological and molecular biology techniques. We will expand these studies in vivo, and determine, by CHIP analysis, if the chromatin structure of the gene is important for MUC2 expression. In addition, we will determine the dynamic occupancy of SP1/SP3 at functionally relevant sites, and the transcription factors and co-factors that may participate in the formation of multiprotein complexes upon interaction with SP1 and SP3 as a function of MUC2 repression. 5. Finally, we will extend our use of microarray technology to determine how NaB perturbs profiles of gene expression that define lineage specific differentiation (Velcich et al., submitted).

Project Information

Project Name or Project/Program Title*: COLON CELL DIFFERENTIATION: NAB AND MUC2 GENE EXPRESSION

Project Status*: Recipient responsible for this data

Total Federal Amount of ARRA Funds Received/Invoiced*: Recipient responsible for this data

Number of Jobs*: Recipient responsible for this data

Description of Jobs Created*: Recipient responsible for this data

Quarterly Activities/Project Description*: Recipient responsible for this data

Activity Code (NAICS or NTEE-NPC)*: Recipient responsible for this data

Total Federal Amount of ARRA Expenditure* (Enter the cumulative total amount of Recovery Funds received that were expended to projects or activities. Refer to the Data Model for details on how to calculate this amount.): Recipient responsible for this data

Total Federal ARRA Infrastructure Expenditure Recipient responsible for this data

Infrastructure Contact Name: Recipient responsible for this data

Infrastructure Contact Email: Recipient responsible for this data

Infrastructure Contact Phone: Recipient responsible for this data

Infrastructure Contact Phone Ext: Recipient responsible for this data

Infrastructure Contact Street Address 1: 111 EAST 210TH STREET

Infrastructure Contact Street Address 2: Not Available

Infrastructure Contact Street Address 3: Recipient responsible for this data

Infrastructure City: BRONX

Infrastructure State: NY

Infrastructure ZIP Code+4: 10467-2401

Infrastructure Purpose and Rationale (If applicable, enter an explanation about how the infrastructure investment will contribute to one or more purposes of the Recovery Act. Refer to the Data Model for details on what to report. 4000 characters or less.): Recipient responsible for this data

Primary Place of Performance

Street Address 1: 111 EAST 210TH ST

Street Address 2: BRONX

City*: BRONX

State*: NY

ZIP Code+4*: 104672490

Congressional District*: Not Available

Country*: US

Recipient Highly Compensated Officers

Prime Recipient Indication of Reporting Applicability*: Recipient responsible for this data

1. Officer Name and Compensation: Recipient responsible for this data
2. Officer Name and Compensation: Recipient responsible for this data
3. Officer Name and Compensation: Recipient responsible for this data
4. Officer Name and Compensation: Recipient responsible for this data
5. Officer Name and Compensation: Recipient responsible for this data

This concludes the current search. To begin a new search, return to the HHS Recovery Act Recipient Reporting Readiness Tool Landing Page.