HHS Recovery Act Recipient Reporting Readiness Tool
Step 4. Review and Copy the Grant Awards Data
TAGGS provides some – but not all – of the data needed for the Recipient Report. Recipients are responsible for directly collecting and reporting all required data to FederalReporting.gov. Data that HHS does not currently collect are highlighted in yellow. Do not copy this highlighted information. Please enter the appropriate data for your organization in these required fields. For assistance with entering these data please contact FederalReporting.gov.
You may capture the data HHS does provide by copying data from this screen and pasting it into the reporting format of your choice, such as the Excel spreadsheet template, the XML template, or by logging into the online form. For assistance with copying and pasting these data please e-mail our help desk at Readiness Help.
| Recipient Report: Grant or Loan | ||
| Prime Recipient |
| Reporting Information | ||
| Award Type | Award Number | Final Report |
| Grant | 2R01GM044006-18A1 | Recipient responsible for this data |
| Award Recipient Information | ||
| Recipient DUNS Number | Recipient Account Number | Recipient Congressional District |
| 078861598 | Recipient responsible for this data | 15 |
| Award Information | ||
| Funding Agency Code | Awarding Agency Code | Award Date |
| 7529 | 7529 | 08-28-2009 |
| Amount of Award | Sub Account Number for Program Source (TAS) | |
| $ 367,369 | Recipient responsible for this data | |
| Program Source (TAS)* | CFDA Number | |
| 750852 | 93.701 | |
| Total Number of Sub Awards to Individuals | Total Amount of Sub Awards to Individuals | |
| Recipient responsible for this data | Recipient responsible for this data | |
| Total Number of Payments to Vendors less than $25,000/award | Total Amount of Payments to Vendors less than $25,000/award | |
| Recipient responsible for this data | Recipient responsible for this data | |
| Total Number of Sub Awards less than $25,000/award | Total Amount of Sub Awards less than $25,000/award | |
| Recipient responsible for this data | Recipient responsible for this data | |
| Award Description | ||
| DESCRIPTION (provided by applicant): Protein-DNA selectivity is a central event in many biological processes, ranging from transcription and replication to restriction and modification. Because of the excess of non-specific sequences in a cell the initial encounter between a protein and DNA usually occurs outside of the target sequence. Restriction enzymes are paradigms for the study of protein-DNA selectivity and the intermediates en route to a target sequence. We propose three aims of broad biological significance in understanding the mechanisms for targeting hydrolysis at a specific DNA site. An understanding of sequence specific cleavage is relevant to proteins mediating site specific recombination and DNA repair, and for more effective use of restriction enzymes in basic and clinical applications. 1) We will determine the structures of a BamHI isoschizomer, OkrAI, with and without DNA. OkrAI and BamHI offer an opportunity to compare two enzymes that recognize and cleave exactly the same DNA substrate, even though they differ significantly in secondary structure. 2) Specific versus non-specific DNA binding. We have determined structures of BamHI and BstYI bound to the same non-cognate DNA sequence. We will now determine the structures of BstYI bound to other non-specific DNA sequences, and image BamHI molecules diffusing along DNA. In the presence of certain co-solvents, most restriction enzymes show enhanced cleavage at non-cognate sites, the so-called "star" activity. We will determine the structures of a novel BamHI mutant that maintains fidelity under star conditions. The mutant provides an opportunity to gain a deeper understanding of the structural changes accompanying a switch between non-cognate and cognate DNA binding. 3) We will obtain structural information on EcoP15I, a prototype of ATP-dependent type III family of restriction enzymes. Despite over 30 years of biochemical and genetic studies, there is still no structural information on these multi-subunit enzymes (MW > 350kDa) that consume ATP to acquire directionality on the DNA. We will put a crude understanding of this unique multi-functional ATP-dependent enzyme onto a more firm structural framework. The studies on EcoP15I, BamHI and BstYI complement each other to provide a molecular perspective on active versus passive diffusion in DNA metabolism. PUBLIC HEALTH RELEVANCE: Site-specific hydrolysis of DNA is common to many biological processes. The discovery of restriction enzymes opened the doors of modern biotechnology and they are ideal systems for the study of site-specific hydrolysis. We will uncover the extraordinary mechanisms by which these enzymes target and hydrolyze specific DNA sequences. | ||
| Project Information | ||||||||||||||||||||||||||
| Project Name or Project/Program Title |
Project Status | Total Federal Amount ARRA Funds Received/Invoiced |
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| RECOGNITION AND CLEAVAGE OF DNA BY RESTRICTION ENZYMES | Recipient responsible for this data | Recipient responsible for this data | ||||||||||||||||||||||||
| Number of Jobs | Description of Jobs Created | |||||||||||||||||||||||||
| Recipient responsible for this data | Recipient responsible for this data | |||||||||||||||||||||||||
| Quarterly Activities/Project Description | ||||||||||||||||||||||||||
| Recipient responsible for this data | ||||||||||||||||||||||||||
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| Total Federal Amount of ARRA Expenditure |
Total Federal ARRA Infrastructure Expenditure |
Infrastructure Contact Name | ||||||||||||||||||||||||
| Recipient responsible for this data | Recipient responsible for this data | Recipient responsible for this data | ||||||||||||||||||||||||
| Infrastructure Contact Email | Infrastructure Contact Phone | Infrastructure Contact Phone Ext. | ||||||||||||||||||||||||
| Recipient responsible for this data | Recipient responsible for this data | Recipient responsible for this data | ||||||||||||||||||||||||
| Infrastructure Contact Street Address 1 | Infrastructure Contact Street Address 2 | Infrastructure Contact Street Address 3 | ||||||||||||||||||||||||
| 1 GUSTAVE L LEVY PL, BOX 3500 | Not Available | Recipient responsible for this data | ||||||||||||||||||||||||
| Infrastructure City | Infrastructure State | Infrastructure ZIP Code+4 | ||||||||||||||||||||||||
| NEW YORK-NEW YORK | NY | 10029-6500 | ||||||||||||||||||||||||
| Infrastructure Purpose and Rationale | ||||||||||||||||||||||||||
| Recipient responsible for this data | ||||||||||||||||||||||||||
| Primary Place of Performance | ||
| Street Address 1 | Street Address 2 | City |
| OF NEW YORK UNIVERSITY1 GUSTAVE L. LEVY PL, BOX 1075 | Recipient responsible for this data | NEW YORK |
| State | Zip Code+4 | Congressional District |
| NY | 100296574 | 14 |
| Country | ||
| US | ||
| Recipient Highly Compensated Officers | |||
| Prime Recipient Indication of Reporting Applicability | # | Officer Name | Officer Compensation |
| Recipient responsible for this data | 1 | Recipient responsible for this data | Recipient responsible for this data |
| 2 | Recipient responsible for this data | Recipient responsible for this data | |
| 3 | Recipient responsible for this data | Recipient responsible for this data | |
| 4 | Recipient responsible for this data | Recipient responsible for this data | |
| 5 | Recipient responsible for this data | Recipient responsible for this data | |
This concludes the current search.
To begin a new search, return to the HHS Recovery Act Recipient Reporting Readiness Tool.
USE IN THE RECIPIENT REPORT
The information provided by this tool is baseline data that the Recipient should include in the Recipient Report that must be submitted to FederalReporting.gov beginning October 1, 2009. The data from this tool can be cut and pasted directly into the Recipient Report.
