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HHS Recovery Act Recipient Reporting Readiness Tool

Step 4. Review and Copy the Grant Awards Data

TAGGS provides some – but not all – of the data needed for the Recipient Report. Recipients are responsible for directly collecting and reporting all required data to FederalReporting.gov. Data that HHS does not currently collect are highlighted in yellow. Do not copy this highlighted information. Please enter the appropriate data for your organization in these required fields. For assistance with entering these data please contact FederalReporting.gov.

You may capture the data HHS does provide by copying data from this screen and pasting it into the reporting format of your choice, such as the Excel spreadsheet template, the XML template, or by logging into the online form. For assistance with copying and pasting these data please contact our help desk at 866-814-5703.

 

Award Detail for: 3D HUMAN SKIN MODELS
WISTAR INSTITUTE
DUNS Number: 075524595
36TH AT SPRUCE
PHILADELPHIA, PA 19104
Recipient Report: Grant or Loan
Prime Recipient

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Reporting Information
Award Type Award Number Final Report
Grant 1RC1ES018210-01 Recipient responsible for this data

Award Recipient Information
Recipient DUNS Number Recipient Account Number Recipient Congressional District
075524595 Recipient responsible for this data 1

Award Information
Funding Agency Code Awarding Agency Code Award Date
7529 7529 09-21-2009
Amount of Award Sub Account Number for Program Source (TAS)  
$ 487,225 Recipient responsible for this data
Program Source (TAS)* CFDA Number 
750863 93.701
Total Number of Sub Awards to Individuals Total Amount of Sub Awards to Individuals
Recipient responsible for this data Recipient responsible for this data
Total Number of Payments to Vendors less than $25,000/award Total Amount of Payments to Vendors less than $25,000/award
Recipient responsible for this data Recipient responsible for this data
Total Number of Sub Awards less than $25,000/award Total Amount of Sub Awards less than $25,000/award
Recipient responsible for this data Recipient responsible for this data
Award Description
DESCRIPTION (provided by applicant): This project addresses 3D or virtual models to reduce the use of animals in research: Creation of miniature multi-cellular organs for high-throughput screening for chemical toxicity testing. Our long-term objective is to decrease the use of animals for toxicity assays and increase the use of human cells for toxicity and safety assessment. We will establish a three-dimensional (3D) model of the normal human skin to create experimental conditions that are more 'human-specific' and more predictive with the added benefit of decreasing the number of animals required for preclinical toxicology and safety assessment. Mouse skin is significantly different from human skin in its architecture and varies in response to environmental toxins (biological origin) and xenobiotics (synthetic chemicals). Our working hypothesis is that 3D reconstructed human skin can be used as a reliable system to screen for the effects of xenobiotic exposures. The murine epidermis is very thin and largely devoid of melanocytes, whereas human epidermis is thick and contains pigment-producing melanocytes. Major carcinogens for mouse skin such as DMBA and the phorbol ester PMA/TPA commonly used in classical two-stage carcinogenesis studies in mice show little effect on human skin grafted to immunodeficient animals. To better determine which xenobiotics are affecting human cells, we will reconstruct in vitro human skin containing two layers: 1) 'dermis' consisting of fibroblasts and, optionally, also endothelial, smooth muscle, and inflammatory, and immune cells that are all embedded in collagen type I; 2) 'epidermis' consisting of multilayered keratinocytes at distinct differentiation stages and melanocytes, which reside in the basal layer and attach to the developing basement membrane. Synthetic human skin (also termed skin reconstructs or organotypic skin) contains at least three cell types (fibroblasts, melanocytes, keratinocytes) and up to six additional cell types (e.g., including endothelial cells, smooth muscle cells, and inflammatory cells), and the structures and functions generated are similar to intact, full-thickness human skin. Our specific objectives are: Specific Aim 1. Test human skin reconstructs of various complexities and develop parameters for detecting skin cell damage after exposure to xenobiotics. As parameters for measures of toxicity, we will use cell viability or death, changes in differentiation patterns for keratinocytes, melanocytes, and endothelial cells, and for long-term effects changes in markers associated with transformation. Specific Aim 2. Miniaturize reconstructs to 96-well format and establish standardized criteria for medium-to-high throughput testing. The human skin reconstructs will be established in 96-well plate format and we will perform side-by-side comparison of the in vitro models with the orthotropic human and the murine skin models to compare sensitivity, reproducibility, and interpretability of large-scale 3D testing of xenobiotics. Public Health Relevance Statement These studies aim to establish a three-dimensional (3D) model of the normal human skin to create experimental conditions that are more 'human-specific' and more predictive with the added benefit of decreasing the number of animals required for preclinical toxicology and safety assessment. We will perform side-by-side comparison of the in vitro models with the orthotropic human and the murine skin models to compare sensitivity, reproducibility, and interpretability of large-scale 3D testing of xenobiotics.

Project Information
Project Name or
Project/Program Title
Project Status Total Federal Amount ARRA Funds
Received/Invoiced
3D HUMAN SKIN MODELS Recipient responsible for this data Recipient responsible for this data
Number of Jobs Description of Jobs Created
Recipient responsible for this data Recipient responsible for this data
Quarterly Activities/Project Description
Recipient responsible for this data
 
Activity Code (NAICS or NTEE-NPC)
1Recipient responsible for this data2Recipient responsible for this data
3Recipient responsible for this data4Recipient responsible for this data
5Recipient responsible for this data6Recipient responsible for this data
7Recipient responsible for this data8Recipient responsible for this data
9Recipient responsible for this data10Recipient responsible for this data
Total Federal Amount of ARRA
Expenditure
Total Federal ARRA
Infrastructure Expenditure
Infrastructure Contact Name
Recipient responsible for this data Recipient responsible for this data Recipient responsible for this data
Infrastructure Contact Email Infrastructure Contact Phone Infrastructure Contact Phone Ext.
Recipient responsible for this data Recipient responsible for this data Recipient responsible for this data
Infrastructure Contact Street Address 1 Infrastructure Contact Street Address 2 Infrastructure Contact Street Address 3
36TH AT SPRUCE Not Available Recipient responsible for this data
Infrastructure City Infrastructure State Infrastructure ZIP Code+4
PHILADELPHIA PA 19104
Infrastructure Purpose and Rationale
Recipient responsible for this data

Primary Place of Performance
Street Address 1 Street Address 2 City
Not Available Recipient responsible for this data PHILADELPHIA
State Zip Code+4 Congressional District
PA 191044265 2
Country  
US

Recipient Highly Compensated Officers
Prime Recipient Indication of Reporting Applicability # Officer Name Officer Compensation
Recipient responsible for this data 1 Recipient responsible for this data Recipient responsible for this data
2 Recipient responsible for this data Recipient responsible for this data
3 Recipient responsible for this data Recipient responsible for this data
4 Recipient responsible for this data Recipient responsible for this data
5 Recipient responsible for this data Recipient responsible for this data

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USE IN THE RECIPIENT REPORT

The information provided by this tool is baseline data that the Recipient should include in the Recipient Report that must be submitted to FederalReporting.gov beginning October 1, 2009. The data from this tool can be cut and pasted directly into the Recipient Report.